Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2864 |
| PDBID: | 6TEJ |
| Chains: | C_B |
| Organism: | Mycolicibacterium thermoresistibile, synthetic construct |
| Method: | XRD |
| Resolution (Å): | 2.70 |
| Reference: | 10.1038/s41586-020-2136-9 |
| Antibody | |
| Antibody: | Syb_NL5 synthetic-Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Drug ABC transporter ATP-binding protein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: C
Mutation: NULL
| >6TEJ_C|Chain C|Syb_NL5|synthetic construct (32630) GPSQVQLVESGGGLVQAGGSLRLSCAASGFPVSSSTMTWYRQAPGKEREWVAAINSYGYYTVYADSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCNVKDTGQMRESYDYWGQGTQVTVSA |
Antigen
Chain: B
Mutation: NULL
| >6TEJ_B|Chain B|Drug ABC transporter ATP-binding protein|Mycolicibacterium thermoresistibile (1797) MIRTLLRLVPAEKRGAVAGYAVLTLLSVLLRAVGAVLLIPLLAALFSDTPSDAWLWLGWLTAVTLAGWVTDTNTARLGFDLGFAVLSRTQHDMADRLPNVAMSWFTPDNTATARQAIAATGPELAGLVVNLLTPLIGAALLPAAIGVALLFVSVPLGLAALAGVAVLFGALALSGRLSRAADKVAGETNSAFTERIIEFARTQQALRAARRVEPARSQVGSALAAQHGAGLRLLTMQIPGQVLFSLAGQVALIGFAGMAVWLTVRGQLGVPEAIALIVVLVRYLEPFAAIADLAPALETTRATLNRIQAVLDAPTLPAGRRRLDRTGAAPSIEFDDVRFSYGDEVVLDGVSFTLRPGNTTAIVGPSGSGKTTILSLIAGLQQPASGRVLLDGVDVTTLDPEARRAAVSVVFQHPYLFDGTLRDNVLVGDPEADPDDVTAAMRLARVDELLDRLPDGDATVVGEGGTALSGGERQRVSIARALLKPAPVLLVDEATSALDNANEAAVVDALTADPRPRTRVIVAHRLASIRHADRVLFVEAGRVVEDGAIDELLAAGGRFAQFWAQQQAASEWAIGSTARALEVLFQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: THR33 THR35 TYR37 GLU44 ARG45 GLU46 TRP47 ALA50 ILE51 ASN52 SER53 TYR54 TYR56 TYR57 THR58 VAL59 TYR60 LYS65 LYS99 THR101 GLY102 MET104 ARG105 GLU106 SER107 ASP109 B: ARG325 GLY327 ALA328 ARG355 GLY357 ASN358 ASP433 ASP436 ALA439 ALA440 ARG442 LEU443 LEU482 ASP508 THR511 ALA512 ASP513 PRO514 ARG515 PRO516 ARG530 HIS531 ALA532 ASP533 ALA548 ILE549 ASP550 LEU553 TRP563 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
