Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2846 |
| PDBID: | 6SV2 |
| Chains: | HL_A |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.30 |
| Reference: | 10.1038/s42003-020-01126-6 |
| Antibody | |
| Antibody: | ICSM 18 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human prion protein (PrP) |
| Antigen mutation: | Yes |
| Durg Target: | P04156 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6SV2_H|Chain B[auth H]|ICSM 18-ANTI-PRP THERAPEUTIC FAB HEAVY CHAIN|Mus musculus (10090) EVQLQQSGPELVKPGSSVKISCKASRNTFTDYNLDWVKQSHGKTLEWIGNVYPNNGVTGYNQKFRGKATLTVDKSSSTAYMELHSLTSEDSAVYYCALYYYDVSYWGQGTLVTVSSAKTTPPSVYPLAPGSAAQTNSVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSQSVTCNVAHPASSTAVDKKIAPA |
Light Chain: L
Mutation: NULL
| >6SV2_L|Chain A[auth L]|ICSM 18-ANTI-PRP THERAPEUTIC FAB LIGHT CHAIN|Mus musculus (10090) QIVLTQSPAIMSASPGEKVTMTCSASSSVSYMHWYQQKSGTSPKRWIYDTSKLASGVPARFSGSGSGTSYSLTISSMEAEDAATYFCHQWRSNPYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTGGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNE |
Antigen
Chain: A
Mutation: G9V
| >6SV2_A|Chain C[auth A]|Major prion protein|Homo sapiens (9606) GAVVGGLGVYMLGSAMSRPIIHFGSDYEDRYYRENMHRYPNQVYYRPMDEYSNQNNFVHDCVNITIKQHTVTTTTKGENFTETDVKMMERVVEQMCITQYERESQAYYQRGSS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: SER30 TYR31 HIS33 ASP49 LYS52 TRP90 ARG91 SER92 TYR95 H: THR30 ASP31 TYR32 ASN33 ASP35 ASN50 TYR52 ASN54 ASN55 VAL57 LYS74 TYR99 TYR100 TYR101 A: HIS140 GLY142 SER143 ASP144 TYR145 GLU146 ASP147 ARG148 TYR149 ARG151 GLU152 ASN153 HIS155 ARG156 GLY195 GLU196 ASN197 THR199 THR201 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
