Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2844 |
| PDBID: | 6SSP |
| Chains: | L_H |
| Organism: | Dickeya chrysanthemi, Lama glama |
| Method: | XRD |
| Resolution (Å): | 3.25 |
| Reference: | 10.7554/eLife.51511 |
| Antibody | |
| Antibody: | 21 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Cys-loop ligand-gated ion channel |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: L
Mutation: NULL
| >6SSP_L|Chain K, L|NANOBODY 21|Lama glama (9844) QVQLQESGGGLVQAGGSLRLSCAASGFTFDDYTIGWFRQAPGKEREGVSLISSSLGSTYYADSVKGRITISRDNAKNTVYLQMNSLKPEDTAVYYCAAGRDADPTIFAILRSEYPFDYWGQGTQVTVSSHHHHHHEPEA |
Antigen
Chain: H
Mutation: NULL
| >6SSP_H|Chain A, B, C, D, E, F, G, H, I, J|Cys-loop ligand-gated ion channel|Dickeya chrysanthemi (556) GPVDARPVDVSVSIFINKIYGVNTLEQTYKVDGYIVAQWTGKPRKTPGDKPLIVENTQIERWINNGLWVPALEFINVVGSPDTGNKRLMLFPDGRVIYNARFLGSFSNDMDFRLFPFDRQQFVLELEPFSYNNQQLRFSDIQVYTENIDNEEIDEWWIRGKASTHISDIRYDHLSSVQPNQNEFSRITVRIDAVRNPSYYLWSFILPLGLIIAASWSVFWLESFSERLQTSFTLMLTVVAYAFYTSNILPRLPYTTVIDQMIIAGYGSIFAAILLIIFAHHRQANGVEDDLLIQRCRLAFPLGFLAIGCVLVIRGITG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: LEU55 ASP101 ASP103 ILE106 PHE107 ALA108 ILE109 LEU110 SER112 TYR114 PRO115 H: ARG48 LYS49 THR50 PRO51 LEU56 ARG65 TRP66 ILE67 ASN68 ASN69 GLY70 LEU71 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
