Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2819 |
| PDBID: | 6S8D |
| Chains: | PO_C |
| Organism: | Homo sapiens, Ebola virus |
| Method: | EM |
| Resolution (Å): | 3.49 |
| Reference: | 10.1016/j.chom.2020.01.007 |
| Antibody | |
| Antibody: | 1T0227 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Ebola virus enveloped glycoprotein 1 |
| Antigen mutation: | No |
| Durg Target: | Q05320; Q05320; |
Sequence information
Antibody
Heavy Chain: P
Mutation: NULL
| >6S8D_P|Chain B[auth H], K[auth P], L[auth Y]|Heavy chain|Homo sapiens (9606) EVQLVESGGGLVKPGGSLRLSCAASGFTFRNAWMNWVRQAPGKGLEWVGRIKSRADGGPTDYAAPVKGRFTISRDDSKNTLYLQMNSLKSEDTAVYYCTTHVPDYNSAYYWVYWGQGTLVTVSSGSTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTH |
Light Chain: O
Mutation: NULL
| >6S8D_O|Chain A[auth L], G[auth O], H[auth U]|Light chain|Homo sapiens (9606) QSVLTQPPSVSGAPGQRVTISCTGSSSNIGAGYDVYWYQQLPGTAPKLIIYGNNNRPSGVPDRFSASKSGPSASLAITGLQAEDEADYYCQSYDIRLSDNYVFGTGTKVTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: C
Mutation: NULL
| >6S8D_C|Chain C[auth A], I[auth C], J[auth E]|Envelope glycoprotein|Ebola virus (1570291) ETGRSIPLGVIHNSALQVSDVDKLVCRDKLSSTNQLRSVGLNLEGNGVATDVPSATKRWGFRSGVPPKVVNYEAGEWAENCYNLEIKKPDGSECLPAAPDGIRGFPRCRYVHKVSGTGPCAGDFAFHKEGAFFLYDRLASTVIYRGTTFAEGVVAFLILPQAKKDFFSSHPLREPVNATEDPSSGYYSTTIRYQATGFGTNETEYLFEVDNLTYVQLESRFTPQFLLQLNETIYTSGKRSNTTGKLIWKVNPEIDTTIGEWAFWETKKNLTRKIRSEELSFTVVSTHHQDTGEESASSGKLGLITNTIAGVAGLITGGRRTRR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| O: ALA32 GLY33 TYR34 ASP35 TYR37 TYR52 TYR94 ILE96 SER99 ASP100 P: TRP33 ARG50 LYS52 SER53 ASP56 GLY58 PRO59 HIS101 TYR110 C: LYS114 LYS115 PRO116 ASP117 GLY118 SER119 GLY143 THR144 GLY145 PRO146 ALA148 GLN221 THR223 GLY224 THR227 GLU229 GLU231 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
