Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2817 |
| PDBID: | 6S3T |
| Chains: | EI_T |
| Organism: | Mesomycoplasma hyopneumoniae J, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.50 |
| Reference: | 10.1107/S2059798320003903 |
| Antibody | |
| Antibody: | P46 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | immunodominant surface protein P46 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >6S3T_E|Chain D, F[auth E], H[auth N], J[auth R]|Immunoglobulin heavy chain|Mus musculus (10090) MKMFTLLYLLTVVPGILSDVQLQESGPGLVKPSQTVSLTCTVTGISITTGNYRWSWIRQFPGNKLEWMGYISYSDTITYNPSLTSRTTITRDISRNQIFLEMNSLTAEDTATYYCAREGVLHWYFAVWGAGTTVTVSSAKTTPPSVYPLAPGCGDTTGSSVTLGCLVKGYFPESVTVTWNSGSLSSSVHTFPALLQSGLYTMSSSVTVPSSTWPSQTVTCSVAHPASSTTVDKKLEPSGPISTINPCPPCKECHKCPAPNLEGGPSVFIFPPNIKDVLMISLTPKVTCVVVDVSEDDPDVQISWFVNNVEVHTAQTQTHREDYNSTIRVVSTLPIQHQDWMSGKEFKCKVNNKDLPSPIERTISKIKGLVRAPQVYILPPPAEQLSRKDVSLTCLVVGFNPGDISVEWTSNGHTEENYKDTAPVLDSDGSYFIYSKLNMKTSKWEKTDSFSCNVRHEGLKNYYLKKTISRSPGK |
Light Chain: I
Mutation: NULL
| >6S3T_I|Chain C, E[auth I], G[auth M], I[auth Q]|Immunoglobulin light chain|Mus musculus (10090) METDTLLLWVLLLWVPGSTGDIVLTQSPASLVVSLGQRATISCRASQTVSTSSYSYMHWYQQKPGQPPKLLIYYASNLESGVPARFTGSGSGTDFTLNIHPVEEEDTATYYCQHSWDIPPTFGAGTKLELRRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: T
Mutation: NULL
| >6S3T_T|Chain A, B, K[auth S], L[auth T]|46 kDa surface antigen|Mycoplasma hyopneumoniae J (262719) MSGSTSDSKPQAETLKHKVSNDSIRIALTDPDNPRWISAQKDIISYVDETEAATSTITKNQDAQNNWLTQQANLSPAPKGFIIAPENGSGVGTAVNTIADKGIPIVAYDRLITGSDKYDWYVSFDNEKVGELQGLSLAAGLLGKEDGAFDSIDQMNEYLKSHMPQETISFYTIAGSQDDNNSQYFYNGAMKVLKELMKNSQNKIIDLSPEGENAVYVPGWNYGTAGQRIQSFLTINKDPAGGNKIKAVGSKPASIFKGFLAPNDGMAEQAITKLKLEGFDTQKIFVTGQDYNDKAKTFIKDGDQNMTIYKPDKVLGKVAVEVLRVLIAKKNKASRSEVENELKAKLPNISFKYDNQTYKVQGKNINTILVSPVIVTKANVDNPDALE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| I: THR31 SER32 SER33 TYR34 TYR36 TYR54 ASN57 SER95 TRP96 ASP97 ILE98 E: ASN33 ARG35 TYR52 SER54 TYR55 SER56 THR58 THR60 VAL102 LEU103 HIS104 TRP105 T: ASP63 TYR253 GLY296 GLU299 GLN300 THR303 LYS304 LYS306 LEU307 GLY309 PHE310 ASP311 GLN313 LYS314 ASP324 LYS325 THR328 PHE329 LYS331 ASP332 ASP334 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
