Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2802 |
| PDBID: | 6RPS |
| Chains: | HL_A |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.79 |
| Reference: | 10.1016/j.jmb.2019.10.022 |
| Antibody | |
| Antibody: | 6A10 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Carbonic anhydrase 12 |
| Antigen mutation: | No |
| Durg Target: | O43570 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6RPS_H|Chain D[auth H], F[auth N]|Fab Heavy chain|Homo sapiens (9606) QVQLKESGPGLVQPSETLSLTCTVSGFSLTTYSVSWVRQPSGKGPEWMGRMWYDGDTVYNSALKSRLSISRDTSKNQVFLKMNSLETDETGTYYCTRDFGYFDGSSPFDYWGQGVMVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT |
Light Chain: L
Mutation: NULL
| >6RPS_L|Chain C[auth L], E[auth M]|Fab Light chain|Homo sapiens (9606) PVDIVLTQSPATLSVTPGESVSLSCRASQGISTSIHWYQQKSNESPRLLIKFASQSISGIPSRFSGSGSGTDFTLSINRVESEDFSVYFCQQTYSLPYTFGAGTKLELKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >6RPS_A|Chain A, B|Carbonic anhydrase 12|Homo sapiens (9606) MKWTYFGPDGENSWSKKYPSCGGLLQSPIDLHSDILQYDASLTPLEFQGYNLSANKQFLLTNNGHSVKLNLPSDMHIQGLQSRYSATQLHLHWGNPNDPHGSEHTVSGQHFAAELHIVHYNSDLYPDASTASNKSEGLAVLAVLIEMGSFNPSYDKIFSHLQHVKYKGQEAFVPGFNIEELLPERTAEYYRYRGSLTTPPCNPTVLWTVFRNPVQISQEQLLALETALYCTHMDDPSPREMINNFRQVQKFDERLVYTSFSQGGGGSGGGGSGGGGSHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: THR31 SER32 PHE50 GLN53 THR91 TYR92 SER93 LEU94 TYR96 H: SER28 THR30 THR31 TYR32 ARG50 TRP52 TYR53 ASP54 GLY55 ASP56 THR57 VAL58 TYR59 SER61 LYS64 ARG71 THR73 PHE96 GLY97 TYR98 PHE99 A: MET3 LYS4 TRP5 LYS19 TYR20 GLN56 LEU58 THR60 ASN62 HIS64 LYS67 ASN69 THR91 GLN92 HIS94 VAL121 ASP130 ALA131 SER132 SER135 ASN136 LEU141 HIS166 LYS170 GLY171 GLN172 GLU173 PHE175 LEU198 THR200 PRO201 PRO202 ASN204 HIS234 MET235 ASP236 PRO238 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
