Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 280 |
| PDBID: | 2I9L |
| Chains: | BA_I |
| Organism: | Mus musculus, Vaccinia virus |
| Method: | XRD |
| Resolution (Å): | 3.10 |
| Reference: | 10.1016/j.virol.2007.06.042 |
| Antibody | |
| Antibody: | 7D11 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | poxvirus membrane protein M25 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >2I9L_B|Chain B, D, F, H|Antibody 7D11 heavy chain|Mus musculus (10090) QVQLQQSGAELAKPGASVKMSCKASGYTFTRYWMHWVKQRPGQGLEWIGYINPSTGYTEYNQKFKDKATLTADKSSSTVYMQLSSLTSEDSAVYYCARTTVDGYDFAYWGQGTLVTVSAAKTTAPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPR |
Light Chain: A
Mutation: NULL
| >2I9L_A|Chain A, C, E, G|Antibody 7D11 light chain|Mus musculus (10090) DMVMSQSPSSLAVSAGEKVSMSCKSSQTLLNSRTRKNYLAWYQQKPGQSPKLLIYWASTRESGVPDRFTGSGSGTDFTLTISSVQAEDLAVYYCKQSYNLWTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERAAGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: I
Mutation: NULL
| >2I9L_I|Chain I, J, K, L|Virion membrane protein M25|Vaccinia virus (10245) GAAASIQTTVNTLSERISSKLEQEANASAQTKCDIEIGNFYIRQNHGCNLTVKNMCSADADAQLDAVLSAATETYSGLTPEQKAYVPAMFTAALNIQTSVNTVVRDFENYVKQTCNSSAVVDNKLKIQNVIIDECYGAPGSPTNLEFINTGSSKGNCAIKALMQLTTKATTQIAPKQVAGTGVQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: THR34 LYS36 TYR38 TRP56 SER97 TYR98 LEU100 TRP101 B: THR30 ARG31 TYR32 TRP33 TYR50 ASN52 SER54 THR55 GLY56 TYR57 THR58 GLU59 LYS74 VAL101 ASP102 GLY103 TYR104 I: GLU25 ALA26 ASN27 GLN31 THR32 LYS33 CYS34 ASP35 ILE36 SER58 ALA59 ASP60 ALA61 ASP62 ALA63 VAL122 ASN124 LYS125 LYS127 SER153 LYS155 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
