Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2785 |
| PDBID: | 6RCU |
| Chains: | BC_A |
| Organism: | Plasmodium falciparum 3D7, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 4.00 |
| Reference: | 10.1016/j.cell.2019.05.025 |
| Antibody | |
| Antibody: | R5.004 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Reticulocyte binding protein homologue 5 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >6RCU_B|Chain B|R5.004 heavy chain|Homo sapiens (9606) VHSEVQLVQSGAEVKKPGSSVKVSCKASGGTFSNYAINWVRQAPGQGLEWMGGIIPIFATTNYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYFCARDKHSWSYAFDIWGQGTMVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTC |
Light Chain: C
Mutation: NULL
| >6RCU_C|Chain C|R5.004 light chain|Homo sapiens (9606) SWAQSVLTQPPSASGTPGLRVTISCSGSSSNIGSNTVNWYQHLPGTAPKLLIHSNNQRPSGVPDRFSGSKSGTSASLAISGLQSEDEADYYCAAWDDSLNGWVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: A
Mutation: NULL
| >6RCU_A|Chain A|Reticulocyte binding protein homologue 5|Plasmodium falciparum (isolate 3D7) (36329) ENAIKKTKNQENQLTLLPIKSTEEEKDDIKNGKDIKKEIDNDKENIKTNNAKDHSTYIKSYLNTNVNDGLKYLFIPSHNSFIKKYSVFNQINDGMLLNEKNDVKNNEDYKNVDYKNVNFLQYHFKELSNYNIANSIDILQEKEGHLDFVIIPHYTFLDYYKHLSYNSIYHKSSTYGKCIAVDAFIKKINEAYDKVKSKCNDIKNDLIATIKKLEHPYDINNKNDDSYRYDISEEIDDKSEETDDETEEVEDSIQDTDSNHTPSNKKKNDLMNRTFKKMMDEYNTKKKKLIKCIKNHENDFNKICMDMKNYGTNLFEQLSCYNNNFCNTNGIRYHYDEYIHKLILSVKSKNLNKDLSDMTNILQQSELLLTNLNKKMGSYIYIDTIKFIHKEMKHIFNRIEYHTKIINDKTKIIQDKIKLNIWRTFQKDELLKRILDMSNEYSLFITSDHLRQMLYNTFYSKEKHLNNIFHHLIYVLQMKFNDVPIKMEYFQTYKKNKPLTQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: SER30 ASN31 TYR32 ALA33 ASN35 TRP47 GLY50 ILE52 ILE54 PHE55 THR57 THR58 ASN59 ASP99 HIS101 SER102 TYR105 C: SER26 SER27 SER31 ASN32 THR33 ASN52 LYS67 TRP92 ASP94 SER95 ASN97 TRP99 A: LYS196 SER197 TYR200 TYR346 ASN347 PHE350 ASN352 ASN354 ARG357 TYR358 TYR360 ASP361 GLU362 HIS365 LYS366 LEU369 GLN439 ILE442 LYS443 ILE446 TRP447 ARG448 THR449 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
