Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2780 |
| PDBID: | 6R0X |
| Chains: | AB_E |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.13 |
| Reference: | 10.7554/eLife.46840 |
| Antibody | |
| Antibody: | 6R0X Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Megakaryocyte and platelet inhibitory receptor G6b |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >6R0X_A|Chain A, C|antibody fab fragment heavy chain|Homo sapiens (9606) MKCSWVIFFLMAVVTGVNSEVQLQQSGAELVKPGASVKLSCTASGFNIKETYIHWVKQRPEQGLEWIGRIDPADVYGRYDPKFQGKATITADTSSNSAYLQVSSLTSEDTAVYYCARSYGSSYGIDYWGQGTSVTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDC |
Light Chain: B
Mutation: NULL
| >6R0X_B|Chain B, D|antibody fab fragment light chain|Homo sapiens (9606) MMSSAQFLGLLLLCFQGTRCDIQMTQTTSSLSASLGDRVTISCRASQDISNYLNWYQQKPDGTVKLLIYYTSTLHSGVPSRFSGSGSGTDYSLTISNLEQEDVATYFCQQGYTLPWTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDVNVKWKIDGSERQSGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVTSFNRNEC |
Antigen
Chain: E
Mutation: NULL
| >6R0X_E|Chain E, F|Megakaryocyte and platelet inhibitory receptor G6b|Homo sapiens (9606) NPGASLDGRPGDRVDLSCGGVSHPIRWVWAPSFPACKGLSKGRRPILWAAAAGAPTVPPLQPFVGRLRSLDSGIRRLELLLSAGDSGTFFCKGRHEDESRTVLHVLGDRTYCKAPG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: LYS49 GLU50 THR51 TYR52 HIS54 ARG69 ASP71 ALA73 ASP74 TYR76 ARG78 TYR119 GLY120 SER121 SER122 TYR123 B: GLN47 SER50 TYR52 TYR70 GLY111 TYR112 THR113 LEU114 TRP116 E: GLY20 ALA21 SER22 LEU23 ASP24 GLY25 ARG26 ASP29 VAL31 ASP32 ARG93 VAL119 HIS121 LEU123 GLY124 ASP125 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
