Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2777 |
| PDBID: | 6QX4 |
| Chains: | C_A |
| Organism: | Bacillus anthracis, Lama glama |
| Method: | XRD |
| Resolution (Å): | 3.27 |
| Reference: | 10.1038/s41564-019-0499-1 |
| Antibody | |
| Antibody: | NbAF683 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | S-layer protein sap |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: C
Mutation: NULL
| >6QX4_C|Chain C, E|Nanobody NbAF683|Lama glama (9844) QVQLVESGGGLVQAGGSLRLSCAASGSIFRINDMGWYRQAPGKQRELVAAITSGGSTNYADSVKGRFTISRDNAKKMVYLQMNSLKPEDTAVYYCHADFSTGWAPYDYWGQGTQVTVSSHHHHHHEPEA |
Antigen
Chain: A
Mutation: NULL
| >6QX4_A|Chain A, B|S-layer protein sap|Bacillus anthracis (1392) MSAKAVTTQKVEVKFSKAVEKLTKEDIKVTNKANNDKVLVKEVTLSEDKKSATVELYSNLAAKQTYTVDVNKVGKTEVAVGSLEAKTIEMADQTVVADEPTALQFTVKDENGTEVVSPEGIEFVTPAAEKINAKGEITLAKGTSTTVKAVYKKDGKVVAESKEVKVSAEGAAVASISNWTVAEQNKADFTSKDFKQNNKVYEGDNAYVQVELKDQFNAVTTGKVEYESLNTEVAVVDKATGKVTVLSAGKAPVKVTVKDSKGKELVSKTVEIEAFAQKAMKEIKLEKTNVALSTKDVTDLKVKAPVLDQYGKEFTAPVTVKVLDKDGKELKEQKLEAKYVNKELVLNAAGQEAGNYTVVLTAKSGEKEAKATLALELKAPGAFSKFEVRGLEKELDKYVTEENQKNAMTVSVLPVDANGLVLKGAEAAELKVTTTNKEGKEVDATDAQVTVQNNSVITVGQGAKAGETYKVTVVLDGKLITTHSFKVVDTAPTAKGLAVEFTSTSLKEVAPNADLKAALLNILSVDGVPATTAKATVSNVEFVSADTNVVAENGTVGAKGATSIYVKNLTVVKDGKEQKVEFDKAVQVAVSIKEAKPATKHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: GLN1 VAL2 SER27 ILE28 PHE29 ARG30 GLN39 GLN44 ARG45 LEU47 ASN58 TYR59 ALA60 ASP61 LYS76 TYR94 PHE99 THR101 GLY102 TRP103 PRO105 TYR106 ASP107 TYR108 TRP109 GLY110 A: THR221 THR222 GLN223 LYS224 LYS238 LEU253 LYS255 GLU269 LEU270 TYR271 SER272 ASN273 ALA276 LEU297 ALA316 LEU317 GLN318 PHE319 THR320 GLU328 VAL329 VAL330 SER331 PRO332 GLU333 ILE335 PHE337 ALA347 LYS348 GLY349 THR404 SER405 LYS406 ASP407 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
