Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2771 |
| PDBID: | 6QGX |
| Chains: | B_A |
| Organism: | Escherichia coli O157 |
| Method: | XRD |
| Resolution (Å): | 2.20 |
| Reference: | 10.1007/s10858-019-00250-8 |
| Antibody | |
| Antibody: | F7 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Outer membrane protein assembly factor BamA |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >6QGX_B|Chain B|NanoF7|Lama glama (9844) GPSQVQLVESGGGLVQAGGSLRLSCAASGRTFSRYAMAWFRRAPGKEREFVAAISASAGTIFYTDSVKGRFTISRDHAKNTVSLQMNSLRPEDTAVYYCAAKTGTWATLDRRYDYWGQGTRVTVSA |
Antigen
Chain: A
Mutation: C291S/C301S
| >6QGX_A|Chain A|Outer membrane protein assembly factor BamA|Escherichia coli O157:H7 (83334) MGSSHHHHHHSSGENLYFQHMRNTGSFNFGIGYGTESGVSFQAGVQQDNWLGTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKSYGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFKTDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDDHKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNYDPDYDYESATQDGAKDLSKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWDMGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDKAEQFQFNIGKTW |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: GLU47 PHE48 SER53 SER55 GLY57 THR58 ILE59 PHE60 TYR61 THR62 ASP63 SER64 LYS66 LYS100 THR101 GLY102 THR103 TRP104 ALA105 THR106 LEU107 ARG109 ARG110 A: GLN495 ASP497 ASP498 ASP500 LEU501 ASP503 SER537 ASN538 GLN540 ASP560 GLN561 ASP562 TYR674 PHE675 PRO676 HIS677 GLN678 ALA679 ASP698 LEU699 SER700 LYS701 SER702 ASP703 ASP704 LYS798 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
