Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2770 |
| PDBID: | 6QGW |
| Chains: | B_A |
| Organism: | Escherichia coli O157 |
| Method: | XRD |
| Resolution (Å): | 1.94 |
| Reference: | 10.1007/s10858-019-00250-8 |
| Antibody | |
| Antibody: | E6 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Outer membrane protein assembly factor BamA |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >6QGW_B|Chain B|NanoE6|Lama glama (9844) SQMQLVESGGGLVQAGGSLTLSCAASGRTFSDYDMGWFRQAPGKAREFVARISRSGRMTSLADSVKGRFTISRDNGKRTVYLQMNSLKPEDTAVYYCAADPQWSRVRSGADYWGQGTRVTVSA |
Antigen
Chain: A
Mutation: NULL
| >6QGW_A|Chain A|Outer membrane protein assembly factor BamA|Escherichia coli O157:H7 (83334) MGSSHHHHHHSSGENLYFQHMRNTGSFNFGIGYGTESGVSFQAGVQQDNWLGTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKSYGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFKTDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDDHKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNYDPDYDYESATQDGAKDLSKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWDMGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDKAEQFQFNIGKTW |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: ARG51 SER53 ARG54 SER55 ARG57 MET58 THR59 SER60 LEU61 ASP63 LYS66 THR70 ILE71 TRP103 SER104 A: LEU549 MET552 GLY553 GLU554 HIS555 PRO556 SER557 ASP560 ASP562 ASN563 SER564 PHE565 LYS566 THR567 ASP605 LYS644 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
