Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2763 |
| PDBID: | 6Q6Z |
| Chains: | B_A |
| Organism: | Arabidopsis thaliana, Lama glama |
| Method: | XRD |
| Resolution (Å): | 3.48 |
| Reference: | 10.1016/j.jsb.2019.09.007 |
| Antibody | |
| Antibody: | ENB21 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Protein EDS1L |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >6Q6Z_B|Chain B|EDS1-SPECIFIC NANOBODY|Lama glama (9844) QVQLQESGGGLVQAGGSLRLSCTASGFTFDSYHMGWFRRAPGKEREFVAAVSRVTWLIDIADSVKGRFTISRDNAKNTVYLEMNSLKPEDTAQYFCAASQQRLSRSDVQYDYWGQGTQVTVSSAAAYPYDVPDYGSHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >6Q6Z_A|Chain A|Protein EDS1L|Arabidopsis thaliana (3702) MAFEALTGINGDLITRSWSASKQAYLTERYHKEEAGAVVIFAFQPSFSEKDFFDPDNKSSFGEIKLNRVQFPCMRKIGKGDVATVNEAFLKNLEAVIDPRTSFQASVEMAVRSRKQIVFTGHSSGGATAILATVWYLEKYFIRNPNVYLEPRCVTFGAPLVGDSIFSHALGREKWSRFFVNFVTRFDIVPRITLARKASVEETLPHVLAQLDPRNSSVQESEQRITEFYTSVMRDTSTVANQAVCELTGSAEAILETLSSFLELSPYRPAGTFVFSTEKRLVAVNNSDAILQMLFYTCQASDEQEWSLIPFRSIRDHHSYEELVQSMGMKLFNHLDGENSIESSLNDLGVSTRGRQYVQAALEEEKKRVENQKKIIQVIQQERFLKKLAWIEDEYKPKCQAHKNGYYDSFKVSNEENDFKANVKRAELAGVFDEVLGLLKKCQLPDEFEGDIDWIKLATRYRRLVEPLDIANYHRHLKNEDTGPYMKRGRPTRYIYAQRGYEHHILKPNGMIAEDVFWNKVNGLNLGLQLEEIQETLKNSGSECGSCFWAEVEELKGKPYEEVEVRVKTLEGMLREWITAGEVDEKEIFLEGSTFRKWWITLPKNHKSHSPLRDYMMDEITDTLEHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: VAL2 GLY27 PHE28 THR29 PHE30 ASP35 SER36 TYR37 HIS38 SER57 VAL59 THR62 LEU64 ASN82 ALA83 ASN85 GLN114 TYR115 ASP116 TYR117 A: TRP18 SER19 LYS22 GLN23 TYR25 LEU26 HIS122 PRO213 ARG214 SER216 ARG315 HIS318 SER319 GLU321 GLU322 GLN325 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
