Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2746 |
| PDBID: | 6PZZ |
| Chains: | HL_A |
| Organism: | Influenza A virus (A/environment/Shanghai/S1439/2013(H7N9)), Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.60 |
| Reference: | 10.1016/j.chom.2019.10.002 |
| Antibody | |
| Antibody: | NA-80 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | H7N9 neuraminidase |
| Antigen mutation: | No |
| Durg Target: | P03472 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6PZZ_H|Chain C[auth F], F[auth H], I, L[auth K]|NA-80 fragment antibody heavy chain|Homo sapiens (9606) QVQLVQSGAEVKRPGASVKVSCKASGYTFISYGISWVRQAPGQGLEWMGWISAYNGNTNYAQNLQGRVTMTTDTSTSTAYMELRSLRSDDTAVYYCARVIPGTAVDYFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSC |
Light Chain: L
Mutation: NULL
| >6PZZ_L|Chain B[auth E], E[auth G], H[auth J], K[auth L]|NA-80 fragment antibody light chain|Homo sapiens (9606) DIVMTQSPSSLSASVGDRVTISCRASQSISSYLNWYQQKPGKAPKLLIYAASSLQSGVPSRFSGSASGTDFTLTISSLQPEDFATYYCQQSYSAPFTFGPGTKVDIERTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >6PZZ_A|Chain A, D[auth B], G[auth C], J[auth D]|Neuraminidase|Influenza A virus (A/environment/Shanghai/S1439/2013(H7N9)) (1347105) LKPGCNCSHSQPETTNTSQTIINNYYNETNITNIQMEERTSRNFNNLTKGLCTINSWHIYGKDNAVRIGESSDVLVTREPYVSCDPDECRFYALSQGTTIRGKHSNGTIHDRSQYRALISWPLSSPPTVYNSRVECIGWSSTSCHDGKSRMSICISGPNNNASAVVWYNRRPVAEINTWARNILRTQESECVCHNGVCPVVFTDGSATGPADTRIYYFKEGKILKWESLTGTAKHIEECSCYGERTGITCTCRDNWQGSNRPVIQIDPVAMTHTSQYICSPVLTDNPRPNDPNIGKCNDPYPGNNNNGVKGFSYLDGANTWLGRTISTASRSGYEMLKVPNALTDDRSKPIQGQTIVLNADWSGYSGSFMDYWAEGDCYRACFYVELIRGRPKEDKVWWTSNSIVSMCSSTEFLGQWNWPDGAKIEYFL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: SER31 TYR32 TYR49 ALA50 SER53 LEU54 GLN55 SER56 H: THR28 SER31 TYR32 TYR53 ARG94 ILE96 PRO97 GLY98 THR99 A: GLN296 ARG327 PRO328 ASN329 ASP330 PRO331 ASN333 ILE334 GLY335 LYS336 ASN338 ASP339 PRO340 TYR341 PRO342 GLY343 ASN344 ASP386 ARG387 LYS390 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
