Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2740 |
| PDBID: | 6PZW |
| Chains: | FE_C |
| Organism: | Influenza A virus (A/environment/Shanghai/S1439/2013(H7N9)), Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.00 |
| Reference: | 10.1016/j.chom.2019.10.002 |
| Antibody | |
| Antibody: | NA-22 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | H7N9 neuraminidase |
| Antigen mutation: | No |
| Durg Target: | P03472 |
Sequence information
Antibody
Heavy Chain: F
Mutation: NULL
| >6PZW_F|Chain C[auth F], F[auth H], H[auth J], L[auth K]|NA-22 fragment antigen binding heavy chain|Homo sapiens (9606) QVQLVESGGGVVQPGRSLRLSCAASGFTFNNYGMHWVRQAPGKGLEWVAVISYDGSNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKDKRFITMILVGPFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSC |
Light Chain: E
Mutation: NULL
| >6PZW_E|Chain B[auth E], E[auth G], G[auth I], K[auth L]|NA-22 fragment antigen binding light chain|Homo sapiens (9606) QSVLTQPASVSGSPGQSITISCTGTSSDVGGYNYVSWHQQHPGKAPKLMIYDVSNRPSGVSNRFSGSKSGNTASLTISGLQTEDEADYYCSSYTSSTTNVFGTGTKVTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: C
Mutation: NULL
| >6PZW_C|Chain A, D[auth B], I[auth C], J[auth D]|Neuraminidase|Influenza A virus (A/environment/Shanghai/S1439/2013(H7N9)) (1347105) LKPGCNCSHSQPETTNTSQTIINNYYNETNITNIQMEERTSRNFNNLTKGLCTINSWHIYGKDNAVRIGESSDVLVTREPYVSCDPDECRFYALSQGTTIRGKHSNGTIHDRSQYRALISWPLSSPPTVYNSRVECIGWSSTSCHDGKSRMSICISGPNNNASAVVWYNRRPVAEINTWARNILRTQESECVCHNGVCPVVFTDGSATGPADTRIYYFKEGKILKWESLTGTAKHIEECSCYGERTGITCTCRDNWQGSNRPVIQIDPVAMTHTSQYICSPVLTDNPRPNDPNIGKCNDPYPGNNNNGVKGFSYLDGANTWLGRTISTASRSGYEMLKVPNALTDDRSKPIQGQTIVLNADWSGYSGSFMDYWAEGDCYRACFYVELIRGRPKEDKVWWTSNSIVSMCSSTEFLGQWNWPDGAKIEYFL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: GLY28 GLY29 TYR30 ASN31 TYR32 LYS66 GLY68 ASN69 TYR91 SER93 C: THR93 ASN95 ASP356 ALA358 ASN359 LYS378 PRO380 ASN381 ASP385 SER389 LYS390 ILE392 GLN393 THR450 GLU451 PHE452 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
