Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2735 |
| PDBID: | 6PZE |
| Chains: | HL_A |
| Organism: | Influenza A virus (A/Shanghai/02/2013(H7N9)), Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.30 |
| Reference: | 10.1016/j.chom.2019.10.002 |
| Antibody | |
| Antibody: | NA-45 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | H7N9 neuraminidase |
| Antigen mutation: | Yes |
| Durg Target: | P03472 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6PZE_H|Chain C[auth H]|NA-45 FAB HEAVY CHAIN|Homo sapiens (9606) DQVQLVESGPGLVKPSETLSLTCTVSGGSISSSSYYWGWIRQPPGKGLEWIGSIYYSGSTYYNPSLKSRVTISVDTSKNQFSLKLSSVTAADTAVYYCARDPGLEWELSVLSNWFDPWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCHHHHHH |
Light Chain: L
Mutation: NULL
| >6PZE_L|Chain B[auth L]|NA-45 FAB LIGHT CHAIN|Homo sapiens (9606) DQSVLTQPPSVSAAPGQKVTISCSGSSSNIGNNYVSWYQQLPGTAPKLLIYDNNKRPSGIPDRFSGSKSGTSATLGITGLQTGDEADYYCGTWDTSLSAYVFATGTKVTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: A
Mutation: Y94H
| >6PZE_A|Chain A|Neuraminidase|Influenza A virus (A/Shanghai/02/2013(H7N9)) (1332244) GSPSRRNFNNLTKGLCTINSWHIYGKDNAVRIGESSDVLVTREPYVSCDPDECRFYALSQGTTIRGKHSNGTIHDRSQYRALISWPLSSPPTVHNSRVECIGWSSTSCHDGKSRMSICISGPNNNASAVVWYNRRPVAEINTWARNILRTQESECVCHNGVCPVVFTDGSATGPADTRIYYFKEGKILKWESLTGTAKHIEECSCYGERTGITCTCRDNWQGSNRPVIQIDPVAMTHTSQYICSPVLTDNPRPNDPNIGKCNDPYPGNNNNGVKGFSYLDGANTWLGRTISTASRSGYEMLKVPNALTDDRSKPIQGQTIVLNADWSGYSGSFMDYWAEGDCYRACFYVELIRGRPKEDKVWWTSNSIVSMCSSTEFLGQWNWPDGAKIEYFL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: GLY29 ASN30 TYR32 ASP50 ASN51 ASN52 LYS53 LYS66 THR93 SER94 H: TYR52 SER54 SER56 TYR58 PRO96 GLY97 LEU98 GLU99 A: ARG118 ASN146 ILE149 HIS150 ASP151 ARG152 PRO197 ASN198 ASN199 ASN221 ILE222 ARG224 ALA246 THR247 GLU276 GLU277 ARG292 ASN294 PRO326 ASN345 ASN346 ASN347 ALA369 ARG371 TYR406 ARG430 PRO431 LYS432 ASP434 LYS435 TRP437 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
