Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2725 |
| PDBID: | 6PV8 |
| Chains: | HI_B |
| Organism: | Homo sapiens, Escherichia coli O11, Mus musculus |
| Method: | EM |
| Resolution (Å): | 3.87 |
| Reference: | 10.1016/j.neuron.2019.07.030 |
| Antibody | |
| Antibody: | 4G9 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | nicotinic acetylcholine receptor beta4 |
| Antigen mutation: | No |
| Durg Target: | P0ABE7 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6PV8_H|Chain F, H|IgG2b Fab heavy chain|Mus musculus (10090) QVQLQQSGPELVRPGVSVKISCKGSGYTFTDYALYWVRQSHAKSLEWIGVINPYSGNTNYNQKFKGKATMTVDKSSSTAYMELARLTSDDSAIYYCASGYDGYPYTMDYWGPGASVTVSSAKTTPPSVYPLAPGCGDTTGSSVTLGCLVKGYFPESVTVTWNSGSLSSSVHTFPALLQSGLYTMSSSVTVPSSTWPSQTVTCSVAHPASSTTVDKKLEP |
Light Chain: I
Mutation: NULL
| >6PV8_I|Chain G, I|Kappa Fab light chain|Mus musculus (10090) QIVLTQSPAIMSASPGEKVTMTCSASSSMTYMHWYQQKSGTSPKRWIYDTSQLASGVPARFRGSGSGTSFSLTISSMEAEDAATYYCQQWSSNPLTFGGGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: B
Mutation: NULL
| >6PV8_B|Chain B, C, E|Fusion protein of Neuronal acetylcholine receptor subunit beta-4 and Soluble cytochrome b562|Homo sapiens (9606) RVANAEEKLMDDLLNKTRYNNLIRPATSSSQLISIKLQLSLAQLISVNEREQIMTTNVWLKQEWTDYRLTWNSSRYEGVNILRIPAKRIWLPDIVLYNNADGTYEVSVYTNLIVRSNGSVLWLPPAIYKSACKIEVKYFPFDQQNCTLKFRSWTYDHTEIDMVLMTPTASMDDFTPSGEWDIVALPGRRTVNPQDPSYVDVTYDFIIKRKPLFYTINLIIPCVLTTLLAILVFYLPSDCGEKMTLCISVLLALTFFLLLISKIVPPTSLDVPLIGKYLMFTMVLVTFSIVTSVCVLNVHHRSPSTHTMAPWVKRCFLHKLPTFLFMKRPGPDSSPARAFPADLEDNWETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYIQKYLSGRFRQDVQEALEGVSFIAQHMKNDDEDQSVVEDWKYVAMVVDRLFLWVFMFVCVLGTVGLFLPPLFQTHAASEGPYAAQRDSAWSHPQFEK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: VAL69 ASN78 GLN81 TYR122 TYR124 I: TYR53 TRP112 SER113 SER114 ASN115 B: ARG1 VAL2 ALA3 LYS8 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
