Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2722 |
| PDBID: | 6PV8 |
| Chains: | FG_D |
| Organism: | Homo sapiens, Escherichia coli O11, Mus musculus |
| Method: | EM |
| Resolution (Å): | 3.87 |
| Reference: | 10.1016/j.neuron.2019.07.030 |
| Antibody | |
| Antibody: | 4G9 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | nicotinic acetylcholine receptor alpha3 |
| Antigen mutation: | No |
| Durg Target: | P0ABE7 |
Sequence information
Antibody
Heavy Chain: F
Mutation: NULL
| >6PV8_F|Chain F, H|IgG2b Fab heavy chain|Mus musculus (10090) QVQLQQSGPELVRPGVSVKISCKGSGYTFTDYALYWVRQSHAKSLEWIGVINPYSGNTNYNQKFKGKATMTVDKSSSTAYMELARLTSDDSAIYYCASGYDGYPYTMDYWGPGASVTVSSAKTTPPSVYPLAPGCGDTTGSSVTLGCLVKGYFPESVTVTWNSGSLSSSVHTFPALLQSGLYTMSSSVTVPSSTWPSQTVTCSVAHPASSTTVDKKLEP |
Light Chain: G
Mutation: NULL
| >6PV8_G|Chain G, I|Kappa Fab light chain|Mus musculus (10090) QIVLTQSPAIMSASPGEKVTMTCSASSSMTYMHWYQQKSGTSPKRWIYDTSQLASGVPARFRGSGSGTSFSLTISSMEAEDAATYYCQQWSSNPLTFGGGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: D
Mutation: NULL
| >6PV8_D|Chain A, D|Fusion protein of Neuronal acetylcholine receptor subunit alpha-3 and Soluble cytochrome b562|Homo sapiens (9606) SEAEHRLFERLFEDYNEIIRPVANVSDPVIIHFEVSMSQLVKVDEVNQIMETNLWLKQIWNDYKLKWNPSDYGGAEFMRVPAQKIWKPDIVLYNNAVGDFQVDDKTKALLKYTGEVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIGSSMNLKDYWESGEWAIIKAPGYKHDIKYNCCEEIYPDITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPTTHTMPSWVKTVFLNLLPRVMFMTRPTSNEGNAQKPRPLYGAELSADLEDNWETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYIQKYLLSALSPEIKEAIQSVKYIAENMKAQNEAKEIQDDWKYVAMVIDRIFLWVFTLVCILGTAGLFLQPLMAREDA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| F: ASP50 ASN71 ASN76 TYR119 ASP120 GLY121 TYR122 PRO123 TYR124 ASP127 G: MET51 THR52 TYR53 HIS55 ARG67 TYR70 ASP71 SER73 GLN74 TRP112 SER113 D: ARG6 LEU7 GLU9 ARG10 LEU11 GLU13 ASP14 ALA23 ASN24 TYR63 LYS64 LYS66 TRP67 ASN68 PRO69 ASP71 TYR72 TYR112 THR113 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
