Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2713 |
| PDBID: | 6PIS |
| Chains: | IM_A |
| Organism: | Mus musculus, Nothocricetulus migratorius |
| Method: | XRD |
| Resolution (Å): | 2.77 |
| Reference: | 10.7554/eLife.50403 |
| Antibody | |
| Antibody: | 6PIS Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Potassium channel subfamily K member 4 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: I
Mutation: NULL
| >6PIS_I|Chain D[auth H], E[auth I]|ANTIBODY FAB FRAGMENT HEAVY CHAIN|Cricetulus migratorius (3122392) QLQLQESGPGLVKPSQSLSLACSVTGFSLSTGGYQWTWIRQFPGKKLEWMGYISYAGGITYNPSLKSRISITRDTSKNQFFLQLNTVTTEDTATHYCARVQYSGYGNAYFDVWGQGIQVTVSSATTTAPSVYPLAPACDSTTSTTNTVTLGCLVKGYFPEPVTVSWNSGALTSGVHTFPSVLHSGLYSLSSSVTVPSSTWPSQTVTCNVAHPASSTKVDKKIVP |
Light Chain: M
Mutation: NULL
| >6PIS_M|Chain C[auth L], F[auth M]|ANTIBODY FAB FRAGMENT LIGHT CHAIN|Cricetulus migratorius (3122392) DIQLTQLPSFLSVSPGDKVTITCKASQNINQYLHWYQQKPEEAPKLLIYGASNLQTGIPSRFSGSGYGTDFSLTINSLDSEDVGTYFCQQGYTPRTFGPGTKLEIKRADAKPTVSIFPPSSEQLGTGSATLVCFVNNFYPKDINVKWKVDGSEKRDGVLQSVTDQDSKDSTYSLSSTLSLTKADYERHNLYTCEVTHKTSTAAIVKTLNRNEC |
Antigen
Chain: A
Mutation: NULL
| >6PIS_A|Chain A, B|Potassium channel subfamily K member 4|Mus musculus (10090) MTTAPQEPPARPLQAGSGAGPAPGRAMRSTTLLALLALVLLYLVSGALVFQALEQPHEQQAQKKMDHGRDQFLRDHPCVSQKSLEDFIKLLVEALGGGANPETSWTQSSQHSSAWNLGSAFFFSGTIITTIGYGNIVLHTDAGRLFCIFYALVGIPLFGMLLAGVGDRLGSSLRRGIGHIEAIFLKWHVPPGLVRSLSAVLFLLIGCLLFVLTPTFVFSYMESWSKLEAIYFVIVTLTTVGFGDYVPGDGTGQNSPAYQPLVWFWILFGLAYFASVLTTIGNWLRAVSRRTRAEMGGLTAQSNSLEVLFQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition I: GLN35 TYR52 SER54 TYR55 ALA56 GLY57 GLY58 ILE59 THR60 TYR102 TYR105 GLY106 M: ILE2 GLN27 ASN28 ASN30 TYR32 TYR67 GLY68 THR69 TYR92 THR93 ARG95 A: PRO56 HIS57 GLN60 LYS63 LYS64 HIS67 GLY68 GLN71 PHE72 ARG74 ASP75 HIS76 PRO77 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
