Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 270 |
| PDBID: | 2HMI |
| Chains: | DC_B |
| Organism: | Human immunodeficiency virus 1, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.80 |
| Reference: | 10.1006/jmbi.1998.2208 |
| Antibody | |
| Antibody: | 28 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HISUBUNIT OF V-1 REVERSE TRANSCRIPTASE |
| Antigen mutation: | No |
| Durg Target: | P03366; P04585; P03367; P04587; P12497; P35963; Q72547; P03369; P12499; |
Sequence information
Antibody
Heavy Chain: D
Mutation: NULL
| >2HMI_D|Chain F[auth D]|FAB FRAGMENT OF MONOCLONAL ANTIBODY 28|Mus musculus (10090) QITLKESGPGIVQPSQPFRLTCTFSGFSLSTSGIGVTWIRQPSGKGLEWLATIWWDDDNRYNPSLKSRLTVSKDTSNNQAFLNMMTVETADTAIYYCAQSAITSVTDSAMDHWGQGTSVTVSSAATTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKI |
Light Chain: C
Mutation: NULL
| >2HMI_C|Chain E[auth C]|FAB FRAGMENT OF MONOCLONAL ANTIBODY 28|Mus musculus (10090) DIQMTQTTSSLSASLGDRVTISCSASQDISSYLNWYQQKPEGTVKLLIYYTSSLHSGVPSAFSGSGSGTDYSLTISNLEPEDFATYYCQQYSKFPWTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVAWAIDGSAAANGVLNSWTDQDSKDSTYSMSSTLTLTADEYEAANSYTCAATHKTSTSPIVKSFNANEC |
Antigen
Chain: B
Mutation: NULL
| >2HMI_B|Chain D[auth B]|HISUBUNIT OF V-1 REVERSE TRANSCRIPTASE|Human immunodeficiency virus 1 (11676) PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPLDEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFKKQNPDIVIYQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWTVQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLSKLLRGTKALTEVIPLTEEAELELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGAHTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTPPLVKLWYQLE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: TYR32 TYR50 TYR91 SER92 PHE94 TRP96 D: SER30 THR31 SER32 GLY33 TRP54 TRP55 ASP56 ASP58 ARG60 ILE102 THR103 SER104 VAL105 THR106 ASP107 SER108 B: GLU194 GLY196 ARG199 THR200 GLU203 GLN222 LYS223 GLU224 PRO225 PRO226 PHE227 LEU228 TRP229 MET230 GLY231 ARG358 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
