Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2683 |
| PDBID: | 6P67 |
| Chains: | EF_I |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.90 |
| Reference: | 10.1038/s41375-019-0531-8 |
| Antibody | |
| Antibody: | 2B8 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Interleukin-7 receptor subunit alpha |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >6P67_E|Chain A, C, E, H|Anti-IL-7R 2B8 Fab heavy chain|Mus musculus (10090) EVQLQQSGPELVKPGASVKMSCKASGYTFSDYYMHWVKQSHGKSLEWIGYIYPDNGGNGYNQKFKGKATLTVDKSSSTVYMELRSLTSEDSALYYCARGTYYDGSYFDYWGQGTTLTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDK |
Light Chain: F
Mutation: NULL
| >6P67_F|Chain B, D, F, L|anti-IL-7R 2B8 Fab light chain|Mus musculus (10090) DIVMTQSHKFMSTLVGDRVSITCKASQDVSTTVAWYQQKPGQSPKLLIYSASYRYTGVPDRFTGSGSGTDFTFTISSVQAEDLAVYYCQQHYSIPRTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: I
Mutation: NULL
| >6P67_I|Chain G, I, J, K|Interleukin-7 receptor subunit alpha|Homo sapiens (9606) GSHMESGYAQNGDLEDAELDDYSFSCYSQLEVNGSQHSLTCAFEDPDVNTTNLEFEICGALVEVKCLNFRKLQEIYFIETKKFLLIGKSNICVKVGEKSLTCKKIDLTTIVKPEAPFDLSVVYREGANDFVVTFNTSHLQKKYVKVLMHDVAYRQEKDENKWTHVNLSSTKLTLLQRKLQPAAMYEIKVRSIPDHYFKGFWSEWSPSYYFRTPEINNSSGEMD |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: SER135 CYS223 F: ASP151 ASN152 ALA153 ALA184 LYS188 HIS189 LYS190 VAL191 ASN210 GLY212 GLU213 I: SER19 PHE20 SER21 GLU40 LYS99 MET144 GLU155 ASN156 LYS157 TRP158 HIS160 ASN162 ARG186 ILE188 ASP190 LYS194 GLY195 PHE196 TRP197 TRP200 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
