Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2681 |
| PDBID: | 6P67 |
| Chains: | AB_K |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.90 |
| Reference: | 10.1038/s41375-019-0531-8 |
| Antibody | |
| Antibody: | 2B8 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Interleukin-7 receptor subunit alpha |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >6P67_A|Chain A, C, E, H|Anti-IL-7R 2B8 Fab heavy chain|Mus musculus (10090) EVQLQQSGPELVKPGASVKMSCKASGYTFSDYYMHWVKQSHGKSLEWIGYIYPDNGGNGYNQKFKGKATLTVDKSSSTVYMELRSLTSEDSALYYCARGTYYDGSYFDYWGQGTTLTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDK |
Light Chain: B
Mutation: NULL
| >6P67_B|Chain B, D, F, L|anti-IL-7R 2B8 Fab light chain|Mus musculus (10090) DIVMTQSHKFMSTLVGDRVSITCKASQDVSTTVAWYQQKPGQSPKLLIYSASYRYTGVPDRFTGSGSGTDFTFTISSVQAEDLAVYYCQQHYSIPRTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: K
Mutation: NULL
| >6P67_K|Chain G, I, J, K|Interleukin-7 receptor subunit alpha|Homo sapiens (9606) GSHMESGYAQNGDLEDAELDDYSFSCYSQLEVNGSQHSLTCAFEDPDVNTTNLEFEICGALVEVKCLNFRKLQEIYFIETKKFLLIGKSNICVKVGEKSLTCKKIDLTTIVKPEAPFDLSVVYREGANDFVVTFNTSHLQKKYVKVLMHDVAYRQEKDENKWTHVNLSSTKLTLLQRKLQPAAMYEIKVRSIPDHYFKGFWSEWSPSYYFRTPEINNSSGEMD |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: ASP31 TYR33 HIS35 TYR50 TYR52 ASN55 GLY56 GLY59 TYR60 TYR101 TYR102 ASP103 GLY104 SER105 TYR106 B: ASP1 SER30 THR31 THR32 TYR49 SER50 TYR53 HIS91 TYR92 SER93 ILE94 PRO95 ARG96 K: ASP114 LEU115 SER116 VAL117 VAL118 TYR119 ARG120 GLU121 GLY122 PHE126 VAL127 THR129 ASN131 LYS167 MET180 PRO202 SER203 TYR204 TYR205 PHE206 ARG207 THR208 PRO209 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
