Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2674 |
| PDBID: | 6P50 |
| Chains: | HL_C |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.90 |
| Reference: | 10.1038/s41375-019-0531-8 |
| Antibody | |
| Antibody: | 4A10 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Interleukin-7 receptor subunit alpha |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6P50_H|Chain B[auth H]|anti-IL-7R 4A10 Fab heavy chain|Mus musculus (10090) QVQLQQPGAELVMPGASVKLSCKASGYTFTSYWMHWVKQRPGEGLEWIGEIDPSDSYTNDNQKFKGKATLTVDKSSSTAYMQLSSLTSEDSAVYYCARRLYSNSYYYAMDYWGQGTSVTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSQTVTCNVAHPASSTKVDKKIVPRDCG |
Light Chain: L
Mutation: NULL
| >6P50_L|Chain C[auth L]|anti-IL-7R 4A10 Fab light chain|Mus musculus (10090) DIQMTQSPSSLSASLGGKVTITCKASQDIKKYIAWYQHKPGKGPRLLIHYTSTLQPGIPSRFSGSGSGRDYSFSISNLEPVDIATYYCLQYDNLLTFGAGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPRDINVKWKIDGSERQNGVLNSWTDQDSKDSTYNMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: C
Mutation: NULL
| >6P50_C|Chain A[auth C]|Interleukin-7 receptor subunit alpha|Homo sapiens (9606) GSHMESGYAQNGDLEDAELDDYSFSCYSQLEVNGSQHSLTCAFEDPDVNTTNLEFEICGALVEVKCLNFRKLQEIYFIETKKFLLIGKSNICVKVGEKSLTCKKIDLTTIVKPEAPFDLSVVYREGANDFVVTFNTSHLQKKYVKVLMHDVAYRQEKDENKWTHVNLSSTKLTLLQRKLQPAAMYEIKVRSIPDHYFKGFWSEWSPSYYFRTPEINNSSGEMD |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: TYR32 TRP33 ASP52 SER54 ASP55 TYR57 TYR101 SER102 ASN103 SER104 TYR105 TYR106 TYR107 L: LYS30 TYR32 TYR91 ASP92 C: GLU52 CYS54 GLY55 ALA56 VAL58 GLU59 VAL60 LYS61 CYS62 GLY83 LYS84 ASN86 CYS88 LYS90 GLU93 LYS94 SER95 CYS98 LYS99 LYS100 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
