Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2672 |
| PDBID: | 6P4B |
| Chains: | HL_C |
| Organism: | Mus musculus, Gallus gallus |
| Method: | XRD |
| Resolution (Å): | 1.90 |
| Reference: | 10.1073/pnas.2005102117 |
| Antibody | |
| Antibody: | HyHEL10 Fab |
| Antibody mutation: | Yes |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Lysozyme C |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: L4F/Y33H/S56N/Y58F
| >6P4B_H|Chain B, E[auth H]|HyHEL10 Fab heavy chain|Mus musculus (10090) DVQFQESGPSLVKPSQTLSLTCSVTGDSITSDHWSWIRKFPGNRLEYMGYVSYSGNTFYNPSLKSRISITRDTSKNQYYLDLNSVTTEDTATYYCANWDGDYWGQGTLVTVSAAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSQTVTCNVAHPASSTKVDKKIVPRDCGSHHHHHH |
Light Chain: L
Mutation: NULL
| >6P4B_L|Chain A, D[auth L]|HyHEL10 Fab light chain|Mus musculus (10090) DIVLTQSPATLSVTPGNSVSLSCRASQSIGNNLHWYQQKSHESPRLLIKYASQSISGIPSRFSGSGSGTDFTLSINSVETEDFGMYFCQQSNSWPYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: C
Mutation: R21Q/ R73E/ C76S/C94S
| >6P4B_C|Chain C, F[auth D]|Lysozyme C|Gallus gallus (9031) KVFGRCELAAAMKRHGLDNYQGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINSRWWCNDGRTPGSENLSNIPCSALLSSDITASVNSAKKIVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: GLN27 SER28 GLY30 ASN31 ASN32 LYS49 TYR50 GLN53 SER91 ASN92 SER93 TRP94 TYR96 H: ASP27 SER28 ILE29 THR30 SER31 ASP32 HIS33 TYR50 SER52 TYR53 SER54 ASN56 PHE58 TRP98 ASP99 C: LYS13 ARG14 HIS15 GLY16 ASP18 ASN19 TYR20 GLN21 GLY22 ARG61 TRP62 TRP63 GLU73 ASN74 SER76 ASN77 THR89 ASN93 LYS96 LYS97 SER100 ASP101 GLY102 ASN103 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
