Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2670 |
| PDBID: | 6P3S |
| Chains: | VW_X |
| Organism: | Homo sapiens, Influenza A virus (A/chicken/Vietnam/32/2004(H5N1)) |
| Method: | XRD |
| Resolution (Å): | 4.00 |
| Reference: | Not available or To be published |
| Antibody | |
| Antibody: | H5.28 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus H5N1 hemagglutinin |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: V
Mutation: NULL
| >6P3S_V|Chain A, C, F, I, M, P, S, V|Human Fab H5.28 heavy chain|Homo sapiens (9606) EVQLVESGGGLVQPGGSLRLSCAASGFTFSTYWMTWVRQAPGKGLEWVANINQDGGEKYFVDSVKGRFTISRDNAKNSLFLQMNTLRAEDTAVYYCARGFLERLLLGRQGAYYYGMDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPK |
Light Chain: W
Mutation: NULL
| >6P3S_W|Chain B, D, G, J, N, Q, T, W|Human Fab H5.28 light chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVSMTCRASQIISSSLNWYQQKPGKAPKLLIYAASNLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPPELTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE |
Antigen
Chain: X
Mutation: NULL
| >6P3S_X|Chain E, H, K, L, O, R, U, X|Hemagglutinin|Influenza A virus (A/chicken/Vietnam/32/2004(H5N1)) (380831) PLILRDCSVAGWLLGNPMCDEFINVPEWSYIVEKANPVNDLCYPGDFNDYEELKHLLSRINHFEKIQIIPKSSWSSHEASLGVSSACPYQGKSSFFRNVVWLIKKNSTYPTIKRSYNNTNQEDLLVLWGIHHPNDAAEQTKLYQNPTTYISVGTSTLNQRLVPRIATRSKVNGQSGRMEFFWTILKPNDAINFESNGNFIAPEYAYKIVKKAAHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| V: PHE30 THR34 TYR35 PHE103 GLU105 ARG106 LEU107 LEU108 LEU109 GLY110 ARG111 GLN112 GLY113 TYR116 GLY118 ASP120 W: SER31 TYR49 ASN53 LEU54 GLN55 SER56 SER67 X: SER65 ALA67 ALA92 PRO94 ASP97 LEU98 PRO101 GLY102 ASP103 PHE104 TYR107 GLU108 ILE222 ALA223 THR224 ARG225 SER226 LYS227 VAL228 ASN229 ARG234 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
