Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2656 |
| PDBID: | 6OTC |
| Chains: | HL_A |
| Organism: | Lake Victoria marburgvirus - Popp, synthetic construct |
| Method: | XRD |
| Resolution (Å): | 1.70 |
| Reference: | 10.1021/acsinfecdis.9b00091 |
| Antibody | |
| Antibody: | H3 synthetic-Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Marburg virus Polymerase cofactor VP35 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6OTC_H|Chain B[auth H]|sFab H3 heavy chain|synthetic construct (32630) EISEVQLVESGGGLVQPGGSLRLSCAASGFNISYYYIHWVRQAPGKGLEWVASIYPYYGYTSYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARGSSWYGAHAFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTSRHHHHHH |
Light Chain: L
Mutation: NULL
| >6OTC_L|Chain C[auth L]|sFab H3 light chain|synthetic construct (32630) SMASDIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQGYYYAYSLITFGQGTKVEIKRTVAAPSVFIFPPSDSQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGECGGSDYKDDDDK |
Antigen
Chain: A
Mutation: NULL
| >6OTC_A|Chain A|Polymerase cofactor VP35|Lake Victoria marburgvirus (strain Popp-67) (33728) GHMSKPNLSAKDLALLLFTHLPGNNTPFHILAQVLSKIAYKSGKSGAFLDAFHQILSEGENAQAALTRLSRTFDAFMGVVPPVIRVKNFQTVPRPCQKSLRAVPPNPTIDKGWVCVYSSEQGETRALKI |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: GLU4 VAL5 TYR35 ARG101 TRP105 ASP112 TYR113 L: TYR49 LEU54 TYR55 SER56 GLY57 A: GLN254 ILE255 SER257 GLU258 GLU260 ARG268 ARG271 THR272 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
