Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2646 |
| PDBID: | 6OS1 |
| Chains: | D_A |
| Organism: | Homo sapiens, Escherichia coli, synthetic construct |
| Method: | XRD |
| Resolution (Å): | 2.79 |
| Reference: | 10.1126/science.aay9813 |
| Antibody | |
| Antibody: | Nb.AT110i1_le synthetic-Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | angiotensin II type 1 receptor (AT1R) |
| Antigen mutation: | No |
| Durg Target: | P30556; P30556; |
Sequence information
Antibody
Chain: D
Mutation: NULL
| >6OS1_D|Chain B[auth D]|Nanobody Nb.AT110i1_le|synthetic construct (32630) QVQLQESGGGLVAAGGSLRLSCAASGNIFDVDIMGWYRQAPGKERELVASITDGGSTNYADSVKGRFTISRDNAKNTVYLAMASLKPEDTAVYYCAAVAYPDIPTYFDYDSDNFYWGQGTQVTVSSLE |
Antigen
Chain: A
Mutation: NULL
| >6OS1_A|Chain A|Type-1 angiotensin II receptor,Soluble cytochrome b562 BRIL fusion protein|Homo sapiens (9606) DYKDDDDKILNSSTEDGIKRIQDDCPKAGRHNYIFVMIPTLYSIIFVVGIFGNSLVVIVIYFYMKLKTVASVFLLNLALADLCFLLTLPLWAVYTAMEYRWPFGNYLCKIASASVSFNLYASVFLLTCLSIDRYLAIVHPMKSRLRRTMLVAKVTCIIIWLLAGLASLPAIIHRNVFFIENTNITVCAFHYESQNSTLPIGLGLTKNILGFLFPFLIILTSYTLIWKALKKAYDLEDNWETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAEIQKNKPRNDDIFKIIMAIVLFFFFSWIPHQIFTFLDVLIQLGIIRDCRIADIVDTAMPITICIAYFNNCLNPLFYGFLGKKFKRYFLQLLKY |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: ILE28 PHE29 ASP30 ASP32 ILE33 MET34 GLY35 TYR37 ARG45 GLU46 LEU47 SER50 ASP53 GLY54 ASN58 ALA96 VAL98 TYR100 PRO101 ASP102 ILE103 PRO104 THR105 TYR106 PHE107 ASP108 TYR109 ASP110 SER111 ASP112 ASN113 PHE114 TRP116 A: ILE53 MET57 LYS60 THR61 ALA63 SER64 LEU67 LEU122 ARG126 ALA129 ILE130 PRO133 MET134 LYS135 ARG137 LEU138 ARG140 TYR215 LEU222 TYR226 LEU228 LYS1230 ASN1231 LYS1232 PRO1233 ARG1234 ASN1235 ILE1238 ILE1241 ILE1245 TYR1302 GLY1303 LEU1305 GLY1306 LYS1307 LYS1308 PHE1309 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
