Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 264 |
| PDBID: | 2H9G |
| Chains: | HL_S |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.32 |
| Reference: | 10.1016/j.jmb.2006.06.042 |
| Antibody | |
| Antibody: | BdF1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Tumor necrosis factor receptor superfamily member 10B (DR5) precursor |
| Antigen mutation: | No |
| Durg Target: | O14763 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >2H9G_H|Chain B, E[auth H]|Fab BdF1, heavy chain|Homo sapiens (9606) EVQLVESGGGLVQPGGSLRLSCAASGFSIGKSGIHWVRQAPGKGLEWVAVIYPHDGNTAYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARRLALVRMWMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHL |
Light Chain: L
Mutation: NULL
| >2H9G_L|Chain A, D[auth L]|Fab BdF1, light chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCRASQDVSTAVAWYQQKPGKAPKLLIYSASFLYSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYTTPPTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: S
Mutation: NULL
| >2H9G_S|Chain C[auth R], F[auth S]|Tumor necrosis factor receptor superfamily member 10B precursor|Homo sapiens (9606) ALITQQDLAPQQRAAPQQKRSSPSEGLCPPGHHISEDGRDCISCKYGQDYSTHWNDLLFCLRCTRCDSGEVELSPCTTTRNTVCQCEEGTFREEDSPEMCRKCRTGCPRGMVKVGDCTPWSDIECVHKES |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: TYR49 SER50 PHE53 LEU54 TYR55 SER56 GLY57 H: GLU1 VAL2 PHE27 SER28 GLY30 LYS31 SER32 HIS53 ARG94 ARG95 LEU96 ALA97 LEU98 VAL99 TYR102 S: GLY26 ILE34 SER35 GLU36 ASP37 GLY38 ARG39 TYR50 THR52 HIS53 ASN55 ASP56 LEU57 LEU58 PHE59 LEU61 ARG62 THR64 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
