Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 258 |
| PDBID: | 2GHW |
| Chains: | D_C |
| Organism: | Severe acute respiratory syndrome-related coronavirus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.30 |
| Reference: | 10.1074/jbc.M603275200 |
| Antibody | |
| Antibody: | 80R scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | SARS Spike glycoprotein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: D
Mutation: NULL
| >2GHW_D|Chain B, D|anti-sars scFv antibody, 80R|Homo sapiens (9606) MAEVQLVQSGGGVVQPGKSLRLSCAASGFAFSSYAMHWVRQAPGKGLEWVAVISYDGSNKYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARDRSYYLDYWGQGTLVTVSSGGGGSGGGGSGGGGSETTLTQSPATLSLSPGERATLSCRASQSVRSNLAWYQQKPGQAPRPLIYDASTRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQRSNWPPTFGQGTKVEVKSGLVPR |
Antigen
Chain: C
Mutation: NULL
| >2GHW_C|Chain A, C|Spike glycoprotein|SARS coronavirus (694009) MADPNITNLCPFGEVFNATKFPSVYAWERKKISNCVADYSVLYNSTFFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAPGQTGVIADYNYKLPDDFMGCVLAWNTRNIDATSTGNYNYKYRYLRHGKLRPFERDISNVPFSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQPYRVVVLSFELLNAPATVSGLVPR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: SER31 TYR32 ALA33 VAL50 SER52 TYR53 ASP54 SER56 ASN57 TYR59 ARG98 ASP99 ARG100 SER101 TYR102 ASP105 ARG150 ARG162 SER163 ASN164 ASP182 SER184 THR185 SER195 GLY196 SER197 GLY198 SER199 ASP202 PHE203 THR204 THR206 SER208 ARG223 SER224 TRP226 C: LYS390 ARG426 SER432 THR433 TYR436 ASN437 LYS439 TYR440 TYR442 PRO469 PRO470 ALA471 LEU472 ASN473 CYS474 TYR475 TRP476 LEU478 ASN479 ASP480 GLY482 TYR484 THR485 THR486 THR487 GLY488 ILE489 TYR491 GLN492 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
