Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2570 |
| PDBID: | 6O3B |
| Chains: | GE_H |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.50 |
| Reference: | 10.1073/pnas.1817246116 |
| Antibody | |
| Antibody: | F6 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | human Wnt/Frizzled (FZD)-7 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: G
Mutation: NULL
| >6O3B_G|Chain B, E[auth G]|Antibody Fab F6, Heavy chain|Homo sapiens (9606) EVQLVESGGGLVQPGGSLRLSCAASGFNIYYYSMHWVRQAPGKGLEWVASIYSSYSYTSYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARSSPGADYGLDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPK |
Light Chain: E
Mutation: NULL
| >6O3B_E|Chain A, D[auth E]|Antibody Fab F6, Light chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQWAYGPFTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: H
Mutation: NULL
| >6O3B_H|Chain C, F[auth H]|Frizzled-7|Homo sapiens (9606) TGSVPDHGFCQPISIPLCTDIAYNQTILPNLLGHTNQEDAGLEVHQFYPLVKVQCSPELRFFLCSMYAPVCTVLDQAIPPCRSLCERARQGCEALMNKFGFQWPERLRCENFPVHGAGEICVGQNTSDGSGGPGGGPTAY |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: TYR49 SER50 SER53 LEU54 TYR55 SER56 TRP91 TYR93 GLY94 G: TYR30 TYR31 TYR32 SER33 TYR52 SER54 TYR55 TYR57 ARG98 SER99 SER100 PRO101 GLY102 ALA103 ASP104 TYR105 ASP108 TYR109 H: ILE54 PRO55 LEU56 HIS73 GLU77 ASP78 GLY80 LEU81 GLU82 VAL83 HIS84 GLN85 PHE86 TYR87 PRO88 LEU89 LYS91 MET135 ASN136 PHE138 GLY139 PHE140 GLN141 PRO143 ARG145 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
