Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2568 |
| PDBID: | 6O3A |
| Chains: | BA_E |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.10 |
| Reference: | 10.1073/pnas.1817246116 |
| Antibody | |
| Antibody: | F7.B Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | human Wnt/Frizzled (FZD)-7 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >6O3A_B|Chain B|Antibody F7.B Fab, Heavy chain|Homo sapiens (9606) EVQLVESGGGLVQPGGSLRLSCAASGFNFSSSSIHWVRQAPGKGLEWVAYIYPSYDYTYYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARGYFYTWGGMDYWGQGTLVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRV |
Light Chain: A
Mutation: NULL
| >6O3A_A|Chain A|Antibody F7.B Fab, Light chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQYYSLFTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE |
Antigen
Chain: E
Mutation: NULL
| >6O3A_E|Chain C[auth E]|Frizzled-7|Homo sapiens (9606) SVPDHGFCQPISIPLCTDIAYNQTILPNLLGHTNQEDAGLEVHQFYPLVKVQCSPELRFFLCSMYAPVCTVLDQAIPPCRSLCERARQGCEALMNKFGFQWPERLRCENFPVHGAGEICVGQNTSDGSGGPGGGPTAY |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: SER30 SER31 ALA32 TYR49 SER50 SER53 ARG66 TYR91 TYR92 SER93 B: SER30 SER31 TYR50 TYR54 TYR56 THR57 TYR58 TYR96 PHE97 TYR98 THR99 E: PRO55 LEU81 HIS84 GLN85 PHE86 TYR87 PRO88 LEU89 LYS91 VAL92 LEU134 MET135 ASN136 LYS137 PHE138 GLY139 PHE140 GLN141 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
