Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2567 |
| PDBID: | 6O39 |
| Chains: | BA_C |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 1.80 |
| Reference: | 10.1073/pnas.1817246116 |
| Antibody | |
| Antibody: | F2.I Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | human Wnt/Frizzled (FZD)-5 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >6O39_B|Chain B|Antibody F2.I Fab, Heavy chain|Homo sapiens (9606) EVQLVESGGGLVQPGGSLRLSCAASGFNIHSSSIHWVRQAPGKGLEWVAATYSSFGSITYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARYHHPFGYALDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPK |
Light Chain: A
Mutation: NULL
| >6O39_A|Chain A|Antibody F2.I Fab, Light chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQGVYLFTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: C
Mutation: NULL
| >6O39_C|Chain C|Frizzled-5|Homo sapiens (9606) SKAPVCQEITVPMCRGIGYNLTHMPNQFNHDTQDEAGLEVHQFWPLVEIQCSPDLRFFLCSMYTPICLPDYHKPLPPCRSVCERAKAGCSPLMRQYGFAWPERMSCDRLPVLGRDAEVLCMDY |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: VAL92 TYR93 LEU94 B: PHE27 ASN28 HIS30 SER31 SER32 SER33 SER52 SER53 PHE54 GLY55 SER56 THR58 ARG94 TYR95 HIS97 PRO98 PHE99 C: PRO39 PHE55 ASN56 HIS57 GLU62 GLY64 LEU65 GLU66 HIS68 GLN69 PHE70 TRP71 PRO72 LEU73 GLU75 MET120 TYR123 GLY124 PHE125 ALA126 PRO128 ARG130 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
