Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2566 |
| PDBID: | 6O1F |
| Chains: | HL_A |
| Organism: | Homo sapiens, Glycine max |
| Method: | XRD |
| Resolution (Å): | 2.15 |
| Reference: | 10.1016/j.cell.2019.09.009 |
| Antibody | |
| Antibody: | hu31A.v11 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Tryptase alpha/beta-1 |
| Antigen mutation: | No |
| Durg Target: | Q15661; P20231; |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6O1F_H|Chain B[auth H]|Heavy Chain hu31A.v11|Homo sapiens (9606) EVQLVESGGGLVQPGGSLRLSCAASGFTFSDYGMVWVRQAPGKGLEWVAFISSGSSTVYYADTMKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCTRRNYDDWYFDVWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT |
Light Chain: L
Mutation: NULL
| >6O1F_L|Chain D[auth L]|Light Chain hu31A.v11|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCSASSSVTYMYWYQQKPGKSPKPWIYRTSDLASGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQHYHSYPLTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >6O1F_A|Chain A|Tryptase alpha/beta-1|Homo sapiens (9606) AGSTHHHHHHDDDDKIVGGQEAPRSKWPWQVSLRVHGPYWMHFCGGSLIHPQWVLTAAHCVGPDVKDLAALRVQLREQHLYYQDQLLPVSRIIVHPQFYTAQIGADIALLELEEPVNVSSHVHTVTLPPASETFPPGMPCWVTGWGDVDNDERLPPPFPLKQVKVPIMENHICDAKYHLGAYTGDDVRIVRDDMLCAGNTRRDSCQGDSGGPLVCKVNGTWLQAGVVSWGEGCAQPNRPGIYTRVTYYLDWIHHYVPKKP |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: PHE50 SER52 GLY53 SER55 THR56 TYR58 ARG95 TYR97 ASP98 ASP99 L: SER29 THR31 TYR32 TYR34 ARG50 ASP53 TYR91 HIS92 SER93 TYR94 A: HIS36 TRP38 GLN50 ASP60 LEU61 ALA62 ALA63 ARG65 LEU82 PRO84 VAL85 SER86 ARG87 GLU107 GLU109 GLU110 PRO111 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
