Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2565 |
| PDBID: | 6NNJ |
| Chains: | UV_G |
| Organism: | Human immunodeficiency virus 1, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.60 |
| Reference: | 10.1038/s41467-019-08658-4 |
| Antibody | |
| Antibody: | CH31 Fv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV-1 BG505 SOSIP.664 gp120 |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: U
Mutation: NULL
| >6NNJ_U|Chain G[auth U]|CH31 scFv heavy chain|Homo sapiens (9606) QVQLVQSGAAVRKPGASVTVSCKFAEDDDYSPYWVNPAPEHFIHFLRQAPGQQLEWLAWMNPTNGAVNYAWYLNGRVTATRDRSMTTAFLEVKSLRSDDTAVYYCARAQKRGRSEWAYAHWGQGTPVVVSSGGLVPR |
Light Chain: V
Mutation: NULL
| >6NNJ_V|Chain H[auth V]|CH31 scFv light chain|Homo sapiens (9606) DIQMTQSPSSLSASLGDRVTITCQASRGIGKDLNWYQQKAGKAPKLLVSDASTLEGGVPSRFSGSGFHQNFSLTISSLQAEDVATYFCQQYETFGQGTKVDIGGGGSGGGGSGGGGS |
Antigen
Chain: G
Mutation: N107A/T301A/A469C
| >6NNJ_G|Chain D[auth G]|Envelope glycoprotein gp120|Human immunodeficiency virus 1 (11676) AENLWVTVYYGVPVWKDAETTLFCASDAKAYETEKHNVWATHACVPTDPNPQEIHLENVTEEFNMWKNNMVEQMHTDIISLWDQSLKPCVKLTPLCVTLQCTNVTNAITDDMRGELKNCSFNMTTELRDKKQKVYSLFYRLDVVQINENQGNRSNNSNKEYRLINCNTSAITQACPKVSFEPIPIHYCAPAGFAILKCKDKKFNGTGPCPSVSTVQCTHGIKPVVSTQLLLNGSLAEEEVMIRSENITNNAKNILVQFNTPVQINCTRPNNNTRKSIRIGPGQAFYATGDIIGDIRQAHCNVSKATWNETLGKVVKQLRKHFGNNTIIRFANSSGGDLEVTTHSFNCGGEFFYCNTSGLFNSTWISNTSVQGSNSTGSNDSITLPCRIKQIINMWQRIGQAMYAPPIQGVIRCVSNITGLILTRDGGSTNSTTETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRCKRRVVGRRRRRR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| U: PRO31 PHE34 HIS35 TRP47 TRP50 THR53 ASN54 GLY55 ALA56 VAL57 ASN58 TYR59 ALA60 TRP61 TYR62 ARG71 ARG73 MET75 V: ASP1 ARG27 ASP32 GLN90 TYR91 GLU96 G: GLU102 HIS105 THR106 ILE109 ILE194 ASN197 THR198 ASN276 THR278 ASN279 ASN280 ALA281 LYS282 HIS352 PHE353 SER365 GLY366 GLY367 ASP368 VAL371 GLN428 ILE430 GLY431 THR455 ARG456 ASP457 GLY458 THR465 GLU466 THR467 GLY472 GLY473 ASP474 ARG476 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
