Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2561 |
| PDBID: | 6NN3 |
| Chains: | HL_A |
| Organism: | Human parvovirus B19, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.22 |
| Reference: | 10.1128/JVI.01732-18 |
| Antibody | |
| Antibody: | 860-55D Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | VP2 of B19 parvovirus |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6NN3_H|Chain B[auth H]|Fab monoclonal antibody 860-55D, heavy chain|Homo sapiens (9606) QVQLQESGPGLVRPSQTLSLSCTVSGASISSGGYYWSWIRQHPGKGLEWIGHIYYSGTVYYNPSLRSRVSISIDNQLSLKVASVTAADTAVYYCARVRVDSVRGAGDNGFDPWGHGTLVTV |
Light Chain: L
Mutation: NULL
| >6NN3_L|Chain C[auth L]|Fab monoclonal antibody 860-55D, light chain|Homo sapiens (9606) AAALTQPLSVSVSPGQTAIFTCSGDNLGDKYVYWFQQRPGQSPALLIYQDNKRPSGIPERFSGSNSGNTATLTISGTQSTDEADYYCQTWDSTVVFGGGTKL |
Antigen
Chain: A
Mutation: NULL
| >6NN3_A|Chain A|VP2 of B19 parvovirus|Human parvovirus B19 (10798) MTSVNSAEASTGAGGGSSNPVKSMWSEGATFSANSVTCTFSRQFLIPYDPEHHYKVFSPAASSCHNASGKEAKVCTISPIMGYSTPWRYLDFNALNLFFSPLEFQHLIENYGSIAPDALTVTISEIAVKDVTDKTGGGVQVTDSTTGRLCMLVDHEYKYPYVLGQGQDTLAPELPIWVYFPPQYAYLTVGDVNTQGISGDSKKLASEESAFYVLEHSSFQLLGTGGTATMSYKFPPVPPENLEGCSQHFYEMYNPLYGSRLGVPDTLGGDPKFRSLTHEDHAIQPQNFMPGPLVNSVSTKEGDSSNTGAGKALTGLSTGTSQNTRISLRPGPVSQPYHHWDTDKYVTGINAISHGQTTYGNAEDKEYQQGVGRFPNEKEQLKQLQGLNMHTYFPNKGTQQYTDQIERPLMVGSVWNRRALHYESQLWSKIPNLDDSFKTQFAALGGWGLHQPPPQIFLKILPQSGPIGGIKSMGITTLVQYAVGIMTVTMTFKLGPRKATGRWNPQPGVYPPHAAGHLPYVLYDPTATDAKQHHRHGYEKPEELWTAKSRVHPL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: ILE53 SER56 GLY57 THR58 VAL59 TYR60 TYR61 ARG66 SER67 ARG68 VAL69 SER70 ILE71 SER72 LYS80 ALA82 VAL102 ARG103 GLY104 L: ASN26 ASP29 SER66 GLY67 ASP91 SER92 THR93 A: TYR48 PRO50 GLU51 PHE57 SER58 PRO59 ALA60 ALA61 SER62 SER63 ILE80 ARG88 ASP133 GLY138 GLN183 GLN195 GLY196 ILE197 SER198 ASP200 LYS202 ILE475 VAL479 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
