Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2560 |
| PDBID: | 6NMV |
| Chains: | HL_S |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.61 |
| Reference: | 10.1080/19420862.2019.1624123 |
| Antibody | |
| Antibody: | 218 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Tyrosine-protein phosphatase non-receptor type substrate 1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6NMV_H|Chain B[auth H]|Fab 218 anti-SIRP-alpha antibody Variable Heavy Chain|Homo sapiens (9606) DVQLVESGGGVVRPGESLTLSCTASGFTFTSSTMNWVRQAPGEGLDWVSSISTSGVITYYADSVKGRATISRDNSKNTLYLRLFSLRADDTAIYYCATDTFDHWGPGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCHHHHHH |
Light Chain: L
Mutation: NULL
| >6NMV_L|Chain C[auth L]|Fab 218 anti-SIRP-alpha antibody Variable Light Chain|Homo sapiens (9606) ALTQPASVSANPGETVKITCFGSSGNYGWFQQKSPGSAPVTVIHYNNKRPSDIPSRFSGSKSGSTGTLTITGVRAEDEAVYFCGAWETGSATFGAGTTLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: S
Mutation: NULL
| >6NMV_S|Chain A[auth S]|Tyrosine-protein phosphatase non-receptor type substrate 1|Homo sapiens (9606) EEELQVIQPDKSVLVAAGETATLRCTATSLIPVGPIQWFRGAGPGRELIYNQKEGHFPRVTTVSDLTKRNNMDFSIRIGAITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSTRHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: THR28 SER31 SER32 THR33 ASN35 SER50 ILE51 SER52 THR53 SER54 VAL56 ILE57 TYR59 ASP99 THR100 PHE101 L: SER24 GLY25 ASN26 TYR45 LYS48 LYS61 GLY63 TRP86 GLU87 THR88 GLY89 SER90 ALA91 S: PHE39 ARG40 GLY41 ALA42 GLY43 PRO44 GLY45 ARG46 GLU47 LEU48 ALA86 GLY87 THR88 TYR90 VAL92 PHE94 PRO99 VAL102 GLU103 SER106 ALA108 GLU111 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
