Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2559 |
| PDBID: | 6NMU |
| Chains: | BA_C |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.55 |
| Reference: | 10.1080/19420862.2019.1624123 |
| Antibody | |
| Antibody: | 115 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Tyrosine-protein phosphatase non-receptor type substrate 1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >6NMU_B|Chain B, E[auth H]|Fab 115 anti-SIRP-alpha antibody Variable Heavy Chain|Homo sapiens (9606) DVQLVESGGGVVRPGESLRLSCAASGFSFSSYAMNWVRQAPGEGLEWVSRINSGGGGTDYAESVKGRFTISRDNSENTLYLQMNSLRAEDTAVYYCAKQYDWNSFFDYWGLGALVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCHHHHHH |
Light Chain: A
Mutation: NULL
| >6NMU_A|Chain A, D[auth L]|Fab 115 anti-SIRP-alpha antibody Variable Light Chain|Homo sapiens (9606) ETVLTQSPATLSVSPGERATLSCRASQTVGSKLAWHQQKPGQAPRLLIYDATNRATGISDRFSGSGSGTDFTLTISSLQTEDSAVYYCQQYYYWPPYRFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: C
Mutation: NULL
| >6NMU_C|Chain C, F[auth S]|Tyrosine-protein phosphatase non-receptor type substrate 1|Homo sapiens (9606) EEELQVIQPDKSVLVAAGETATLRCTATSLIPVGPIQWFRGAGPGRELIYNQKEGHFPRVTTVSDLTKRNNMDFSIRIGAITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSTRHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: LYS32 TYR91 TYR92 TYR93 TRP94 B: SER28 SER30 SER31 TYR32 ALA33 ARG50 ASN52 SER53 GLY54 GLY56 GLY57 SER75 TYR100 ASP101 TRP102 ASN103 SER104 C: ALA17 PHE39 ARG40 PRO44 GLY45 ARG46 GLU47 LEU48 ILE49 ASN51 GLU54 GLY55 HIS56 PHE57 PRO58 ARG59 ALA80 THR82 PRO83 ALA84 ASP85 TYR89 ASP100 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
