Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2555 |
| PDBID: | 6NMS |
| Chains: | HL_S |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.11 |
| Reference: | 10.1080/19420862.2019.1624123 |
| Antibody | |
| Antibody: | 136 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Tyrosine-protein phosphatase non-receptor type substrate 1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6NMS_H|Chain B, E[auth H]|Fab 136 anti-SIRP-alpha antibody Variable Heavy Chain|Homo sapiens (9606) DVQLVESGGGVVRPGESLRLSCAASGFTFSSYDMNWVRQAPGEGLEWVSLISGSGEIIYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKENNRYRFFDDWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCHHHHHH |
Light Chain: L
Mutation: NULL
| >6NMS_L|Chain A, D[auth L]|Fab 136 anti-SIRP-alpha antibody Variable Light Chain|Homo sapiens (9606) ETVLTQSPGTLTLSPGERATLTCRASQSVYTYLAWYQEKPGQAPRLLIYGASSRATGIPDRFSGSGSGTEFTLTISSLQSEDFAVYYCQQYYDRPPLTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: S
Mutation: NULL
| >6NMS_S|Chain C, F[auth S]|Tyrosine-protein phosphatase non-receptor type substrate 1|Homo sapiens (9606) EEELQVIQPDKSVLVAAGETATLRCTATSLIPVGPIQWFRGAGPGRELIYNQKEGHFPRVTTVSDLTKRNNMDFSIRIGAITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSTRHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: GLN27 TYR30 TYR32 TYR92 ASP93 ARG94 PRO95 H: ASP33 SER52 SER54 GLU56 ILE57 TYR59 GLU99 ASN100 ASN101 ARG102 TYR103 ARG104 S: VAL6 GLN8 PRO9 LYS11 SER12 LEU14 ALA42 GLY43 PRO44 ARG46 GLY87 THR88 TYR90 GLU103 PHE104 LYS105 SER106 GLY107 ALA108 GLY109 GLU111 LEU112 SER113 ARG115 ALA116 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
