Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 255 |
| PDBID: | 2FJH |
| Chains: | BA_W |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.10 |
| Reference: | 10.1074/jbc.M507783200 |
| Antibody | |
| Antibody: | B20-4 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | VEGF-A |
| Antigen mutation: | No |
| Durg Target: | P15692 |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >2FJH_B|Chain C[auth B], F[auth H]|Fab fragment heavy chain|Homo sapiens (9606) EVQLVESGGGLVQPGGSLRLSCAASGFTINASWIHWVRQAPGKGLEWVGAIYPYSGYTNYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARWGHSTSPWAMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTH |
Light Chain: A
Mutation: NULL
| >2FJH_A|Chain B[auth A], E[auth L]|Fab fragment light chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCRASQVIRRSLAWYQQKPGKAPKLLIYAASNLASGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSNTSPLTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: W
Mutation: NULL
| >2FJH_W|Chain A[auth V], D[auth W]|Vascular endothelial growth factor A|Homo sapiens (9606) GQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKD |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: ARG30 ARG31 SER32 TYR49 ASN53 SER91 ASN92 THR93 SER94 LEU96 B: TRP33 HIS35 TRP47 ALA50 TYR52 TYR57 ASN59 TYR60 LYS65 TRP99 HIS101 SER102 THR103 SER104 PRO105 TRP106 W: LYS16 PHE17 MET18 ASP19 TYR21 GLN22 ARG23 TYR25 CYS26 HIS27 ILE29 MET55 CYS61 ASN62 LEU66 ASN100 LYS101 GLU103 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
