Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2547 |
| PDBID: | 6NIU |
| Chains: | HL_Z |
| Organism: | Homo sapiens, Zika virus ZIKV/H. sapiens/FrenchPolynesia/10087PF/2013 |
| Method: | XRD |
| Resolution (Å): | 4.30 |
| Reference: | 10.1038/s41591-019-0746-2 |
| Antibody | |
| Antibody: | MZ4 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | ZIKV E glycoprotein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6NIU_H|Chain A[auth F], G[auth H], I, K[auth M]|Human MZ4 Fab heavy chain|Homo sapiens (9606) QVHLQESGPGLVRPSETLSLTCTVSDGSISSYYWSWIRQPPGKGLEWIGSIYYTGSTNYNPSLKSRVTMSVDTSKNQFSLRLNSVTAADTAMYYCAGLDRYSWNEGGDHWGQGILVSVSS |
Light Chain: L
Mutation: NULL
| >6NIU_L|Chain B[auth G], H[auth J], J[auth L], L[auth N]|Human MZ4 Fab light chain|Homo sapiens (9606) QPVLTQPPSASGTPGQRVSISCSGSRSNLGKNTVNWYQQLPGTAPKLLIYNHSRRPSGVPERFSGSKSGTSASLAISGLQSEDEADYFCAAWDDSLNGLYVFGTGTKVTVL |
Antigen
Chain: Z
Mutation: NULL
| >6NIU_Z|Chain C[auth A], D[auth B], E, F[auth Z]|Envelope protein E|Zika virus (isolate ZIKV/Human/French Polynesia/10087PF/2013) (2043570) IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSGPLEVLFQGPGSAWSHPQFEKGGGSGGGSGGGSAWSHPQFEK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: SER30 SER31 TYR52 TYR53 THR54 ARG97 TYR98 SER99 GLY100 L: GLY29 LYS30 ASN31 THR32 TYR49 ASN50 HIS51 ARG53 LYS66 SER67 GLY68 THR69 Z: ASP37 LYS38 GLY181 GLY182 GLY184 SER185 LYS297 ARG299 LEU300 LYS301 GLY302 VAL303 SER304 TYR305 SER306 THR335 ASP336 GLY337 PRO338 THR366 GLU367 SER368 THR369 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
