Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2525 |
| PDBID: | 6N81 |
| Chains: | CD_B |
| Organism: | Norovirus Hu/GII.4/Farmington Hills/2004/USA, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.58 |
| Reference: | 10.1016/j.immuni.2019.05.007 |
| Antibody | |
| Antibody: | A1227 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Norovirus major capsid protein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >6N81_C|Chain D[auth C], E[auth H]|A1227 Fab heavy chain|Homo sapiens (9606) QVQLVQSGGGMVQPGGSLSLSCAASGFTLSNYAMTWVRQAPGKGLEWVSSIGGSGGTTYYADSVKGRFTISRDSSMNTLYLQMSNLRAGDTAVYYCAKDKTRTLRLGYSGMDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKGLEVLFQ |
Light Chain: D
Mutation: NULL
| >6N81_D|Chain C[auth D], F[auth L]|A1227 Fab light chain|Homo sapiens (9606) DIRLTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPDLLIYGASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFGNYYCQQSFSTPRTFGQGTKVELKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >6N81_B|Chain A, B|Major capsid protein|Norovirus Hu/GII.4/Farmington Hills/2004/USA (1182143) GPSKPFTVPILTVEEMTNSRFPIPLEKLFTGPSGAFVVQPQNGRCTTDGVLLGTTQLSPVNICTFRGDVTHIAGTHNYTMNLASQNWNNYDPTEEIPAPLGTPDFVGRIQGMLTQTTRGDGSTRGHKATVSTGDVHFTPKLGSIQFNTDTNNDFETGQNTKFTPVGVVQDGNGAHQNEPQQWVLPSYSGRTGHNVHLAPAVAPTFPGEQLLFFRSTMPGCSGYPNMNLDCLLPQEWVQHFYQEAAPAQSDVALLRFVNPDTGRVLFECKLHKSGYVTVAHTGQHDLVIPPNGYFRFDSWVNQFYTLAPM |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: SER28 SER30 SER31 TYR32 GLY50 SER67 PHE92 C: VAL2 GLY26 ASN31 TYR32 SER53 THR97 ARG98 THR99 B: LYS225 ASP269 GLY270 VAL271 LEU272 GLU315 GLU316 ILE317 TYR462 GLN463 GLU464 ALA465 PRO467 ALA468 GLN469 SER470 ASP471 LYS493 GLN523 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
