Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2510 |
| PDBID: | 6MXT |
| Chains: | N_A |
| Organism: | Tequatrovirus T4, Homo sapiens, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.96 |
| Reference: | 10.1038/s41589-018-0145-x |
| Antibody | |
| Antibody: | Nb71 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Endolysin, Beta-2 adrenergic receptor chimera |
| Antigen mutation: | No |
| Durg Target: | P07550; P00720; |
Sequence information
Antibody
Chain: N
Mutation: NULL
| >6MXT_N|Chain B[auth N]|nanobody Nb71|Lama glama (9844) QVQLQESGGGLVQPGGSLRLSCAASGFAFSSYELRWYRQAPGKQHELVAGITTGGNTYYADSVKGRFTISRDNAKNTVYLQMSNLRPEDTAVYACNANWDLLSDYWGQGTQVTVSSHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >6MXT_A|Chain A|Endolysin, Beta-2 adrenergic receptor chimera|Enterobacteria phage T4 (10665) GNIFEMLRIDEGLRLKIYKDTEGYYTIGIGHLLTKSPSLNAAKSELDKAIGRNTNGVITKDEAEKLFNQDVDAAVRGILRNAKLKPVYDSLDAVRRAALINMVFQMGETGVAGFTNSLRMLQQKRWDEAAVNLAKSRWYNQTPNRAKRVITTFRTGTWDAYAADEVWVVGMGIVMSLIVLAIVFGNVLVITAIAKFERLQTVTNYFITSLACADLVMGLAVVPFGAAHILTKTWTFGNFWCEFWTSIDVLCVTASIETLCVIAVDRYFAITSPFKYQSLLTKNKARVIILMVWIVSGLTSFLPIQMHWYRATHQEAINCYAEETCCDFFTNQAYAIASSIVSFYVPLVIMVFVYSRVFQEAKRQLQKIDKFCLKEHKALKTLGIIMGTFTLCWLPFFIVNIVHVIQDNLIRKEVYILLNWIGYVNSGFNPLIYCRSPDFRIAFQELLCLRRSSLKAYGNGYSSNGNTGEQSG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| N: GLN1 VAL2 GLY26 PHE27 ALA28 SER31 TYR32 GLU33 ARG35 TYR37 ARG38 GLN39 GLN44 HIS45 LEU47 THR52 THR53 ASN56 THR57 TYR58 TYR59 ALA60 VAL92 ALA94 ASN96 ASN98 TRP99 ASP100 LEU101 LEU102 SER103 ASP104 TYR105 TRP106 GLY107 GLN108 A: GLU1062 ARG1063 GLN1065 THR1066 VAL1067 THR1068 ASN1069 ILE1072 ILE1127 ARG1131 ALA1134 ILE1135 THR1136 PRO1138 PHE1139 TYR1141 GLN1142 SER1143 LYS1147 TYR1219 VAL1222 GLU1225 ARG1228 GLN1229 ILE1233 PHE1264 LEU1266 LYS1267 GLU1268 LYS1270 ALA1271 THR1274 LEU1275 ILE1278 ILE1325 TYR1326 ARG1328 SER1329 PRO1330 ASP1331 ARG1333 ILE1334 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
