Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2508 |
| PDBID: | 6MWX |
| Chains: | KL_J |
| Organism: | Eastern equine encephalitis virus, Mus musculus |
| Method: | EM |
| Resolution (Å): | 8.20 |
| Reference: | 10.1016/j.celrep.2018.11.067 |
| Antibody | |
| Antibody: | EEEV-69 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | chimeric Eastern Equine Encephalitis Virus E2 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: K
Mutation: NULL
| >6MWX_K|Chain C, G, K, O|EEEV-69 antibody heavy chain|Mus musculus (10090) QIQLVQSGREVKNPGETVKISCKASGYTFTEYPMLWVKQAPGKGFRWMGLIYTNTGEPTYAEEFKGRFVFSLEISASTAYLQINNLTNEDTATYFCVRDYFISLDYWGQGTTLTVSSAKTTAPSVYPLAPVCGGTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPALLQSGLYTLSSSVTVTSNTWPSQTITCNVAHPASSTKVDKKIESRR |
Light Chain: L
Mutation: NULL
| >6MWX_L|Chain D, H, L, P|EEEV-69 antibody light chain|Mus musculus (10090) QAVVTQESALTTSPGETVTLTCRSNIGAVTSSNCANWVQEKPDHFFTGLIGDTNNRRSGVPARFSGSLIGDKAALTITGAQTEDEAIYFCALWYNNLWVFGGGTKLTVLGQPKSSPSVTLFPPSSEELETNKATLVCTITDFYPGVVTVDWKVDGTPVTQGMETTQPSKQSNNKYMASSYLTLTARAWERHSSYSCQVTHEGHTVEKSLSRADC |
Antigen
Chain: J
Mutation: NULL
| >6MWX_J|Chain B, F, J, N|E2|Eastern equine encephalitis virus (11021) DLDTHFTQYKLARPYIADCPNCGHSRCDSPIAIEEVRGDAHAGVIRIQTSAMFGLKTDGVDLAYMSFMNGKTQKSIKIDNLHVRTSAPCSLVSHHGYYILAQCPPGDTVTVGFHDGPNRHTCTVAHKVEFRPVGREKYRHPPEHGVELPCNRYTHKRADQGHYVEMHQPGLVADHSLLSIHSAKVKITVPSGAQVKYYCKCPDVREGITSSDHTTTCTDVKQCRAYLIDNKKWVYNSGRLPRGEGDTFKGKLHVPFVPVKAKCIATLAPEPLVEHKHRTLILHLHPDHPTLLTTRSLGSDANPTRQWIERPTTVNFTVTGEGLEYTWGNHPPKRVWAQESGEGNPHGWPHEVVVYYYNRYPLTTIIGLCTCVAIIMVSCVTSVWLLCRTRNLCITPYKLAPNAQVPILLALLCCIKPTRA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| K: PRO2033 TRP2047 LEU2050 TYR2052 THR2055 GLU2057 PRO2058 THR2059 TYR2060 GLU2062 LYS2065 ASP2099 ILE2102 L: GLN2501 ALA2502 SER2532 ASN2533 CYS2534 TRP2593 TYR2594 ASN2595 ASN2596 LEU2597 TRP2598 J: SER2666 ILE2667 HIS2668 SER2669 ALA2670 LYS2671 LYS2673 CYS2688 PRO2689 ASP2690 VAL2691 ARG2692 GLU2693 SER2698 ASP2699 HIS2700 THR2702 THR2703 THR2705 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
