Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2500 |
| PDBID: | 6MWC |
| Chains: | KL_J |
| Organism: | Eastern equine encephalitis virus, Mus musculus |
| Method: | EM |
| Resolution (Å): | 7.50 |
| Reference: | 10.1016/j.celrep.2018.11.067 |
| Antibody | |
| Antibody: | EEEV-5 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | chimeric Eastern Equine Encephalitis Virus E2 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: K
Mutation: NULL
| >6MWC_K|Chain C, G, K, O|EEEV-5 antibody heavy chain|Mus musculus (10090) QIQLVQSGREVKNPGETVKISCKASGYTFTEYPMLWVKQAPGKGFRWMGLIYTNTGEPTYAEEFKGRFVFSLEISASTAYLQINNLTNEDTATYFCVRDYFISLDYWGQGTTLTVSSAKTTAPSVYPLAPVCGGTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPALLQSGLYTLSSSVTVTSNTWPSQTITCNVAHPASSTKVDKKIESRR |
Light Chain: L
Mutation: NULL
| >6MWC_L|Chain D, H, L, P|EEEV-5 antibody light chain|Mus musculus (10090) QAVVTQESALTTSPGETVTLTCRSNIGAVTSSNCANWVQEKPDHFFTGLIGDTNNRRSGVPARFSGSLIGDKAALTITGAQTEDEAIYFCALWYNNLWVFGGGTKLTVLGQPKSSPSVTLFPPSSEELETNKATLVCTITDFYPGVVTVDWKVDGTPVTQGMETTQPSKQSNNKYMASSYLTLTARAWERHSSYSCQVTHEGHTVEKSLSRADC |
Antigen
Chain: J
Mutation: NULL
| >6MWC_J|Chain B, F, J, N|E2|Eastern equine encephalitis virus (11021) DLDTHFTQYKLARPYIADCPNCGHSRCDSPIAIEEVRGDAHAGVIRIQTSAMFGLKTDGVDLAYMSFMNGKTQKSIKIDNLHVRTSAPCSLVSHHGYYILAQCPPGDTVTVGFHDGPNRHTCTVAHKVEFRPVGREKYRHPPEHGVELPCNRYTHKRADQGHYVEMHQPGLVADHSLLSIHSAKVKITVPSGAQVKYYCKCPDVREGITSSDHTTTCTDVKQCRAYLIDNKKWVYNSGRLPRGEGDTFKGKLHVPFVPVKAKCIATLAPEPLVEHKHRTLILHLHPDHPTLLTTRSLGSDANPTRQWIERPTTVNFTVTGEGLEYTWGNHPPKRVWAQESGEGNPHGWPHEVVVYYYNRYPLTTIIGLCTCVAIIMVSCVTSVWLLCRTRNLCITPYKLAPNAQVPILLALLCCIKPTRA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition K: GLU4031 TYR4032 PRO4033 ASP4099 TYR4100 PHE4101 ILE4102 L: GLN4501 ILE4526 THR4530 SER4531 SER4532 ASN4533 ASP4552 ASN4554 ASN4555 TYR4594 ASN4595 ASN4596 J: LYS2543 THR2544 ASP2545 GLY2546 VAL2547 ASN2556 GLY2557 LYS2558 THR2559 GLN2560 LYS2561 SER2562 ILE2563 THR2675 VAL2676 PRO2677 SER2678 SER2697 ASP2716 LYS2718 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
