Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2497 |
| PDBID: | 6MW9 |
| Chains: | OP_N |
| Organism: | Eastern equine encephalitis virus, Mus musculus |
| Method: | EM |
| Resolution (Å): | 7.30 |
| Reference: | 10.1016/j.celrep.2018.11.067 |
| Antibody | |
| Antibody: | EEEV-3 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | chimeric Eastern Equine Encephalitis Virus E2 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: O
Mutation: NULL
| >6MW9_O|Chain C, G, K, O|EEEV-3 antibody heavy chain|Mus musculus (10090) QIQLVQSGREVKNPGETVKISCKASGYTFTEYPMLWVKQAPGKGFRWMGLIYTNTGEPTYAEEFKGRFVFSLEISASTAYLQINNLTNEDTATYFCVRDYFISLDYWGQGTTLTVSSAKTTAPSVYPLAPVCGGTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPALLQSGLYTLSSSVTVTSNTWPSQTITCNVAHPASSTKVDKKIESRR |
Light Chain: P
Mutation: NULL
| >6MW9_P|Chain D, H, L, P|EEEV-3 antibody light chain|Mus musculus (10090) QAVVTQESALTTSPGETVTLTCRSNIGAVTSSNCANWVQEKPDHFFTGLIGDTNNRRSGVPARFSGSLIGDKAALTITGAQTEDEAIYFCALWYNNLWVFGGGTKLTVLGQPKSSPSVTLFPPSSEELETNKATLVCTITDFYPGVVTVDWKVDGTPVTQGMETTQPSKQSNNKYMASSYLTLTARAWERHSSYSCQVTHEGHTVEKSLSRADC |
Antigen
Chain: N
Mutation: NULL
| >6MW9_N|Chain B, F, J, N|E2|Eastern equine encephalitis virus (11021) DLDTHFTQYKLARPYIADCPNCGHSRCDSPIAIEEVRGDAHAGVIRIQTSAMFGLKTDGVDLAYMSFMNGKTQKSIKIDNLHVRTSAPCSLVSHHGYYILAQCPPGDTVTVGFHDGPNRHTCTVAHKVEFRPVGREKYRHPPEHGVELPCNRYTHKRADQGHYVEMHQPGLVADHSLLSIHSAKVKITVPSGAQVKYYCKCPDVREGITSSDHTTTCTDVKQCRAYLIDNKKWVYNSGRLPRGEGDTFKGKLHVPFVPVKAKCIATLAPEPLVEHKHRTLILHLHPDHPTLLTTRSLGSDANPTRQWIERPTTVNFTVTGEGLEYTWGNHPPKRVWAQESGEGNPHGWPHEVVVYYYNRYPLTTIIGLCTCVAIIMVSCVTSVWLLCRTRNLCITPYKLAPNAQVPILLALLCCIKPTRA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition O: TYR5032 PRO5033 LEU5050 TYR5052 ASP5099 TYR5100 PHE5101 ILE5102 SER5103 P: SER5531 SER5532 CYS5534 ASP5552 TRP5593 ASN5595 TRP5598 N: LEU3665 SER3666 ILE3667 HIS3668 SER3669 ALA3670 LYS3671 LYS3673 THR3702 THR3705 ASP3706 VAL3707 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
