Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2495 |
| PDBID: | 6MW9 |
| Chains: | GH_F |
| Organism: | Eastern equine encephalitis virus, Mus musculus |
| Method: | EM |
| Resolution (Å): | 7.30 |
| Reference: | 10.1016/j.celrep.2018.11.067 |
| Antibody | |
| Antibody: | EEEV-3 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | chimeric Eastern Equine Encephalitis Virus E2 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: G
Mutation: NULL
| >6MW9_G|Chain C, G, K, O|EEEV-3 antibody heavy chain|Mus musculus (10090) QIQLVQSGREVKNPGETVKISCKASGYTFTEYPMLWVKQAPGKGFRWMGLIYTNTGEPTYAEEFKGRFVFSLEISASTAYLQINNLTNEDTATYFCVRDYFISLDYWGQGTTLTVSSAKTTAPSVYPLAPVCGGTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPALLQSGLYTLSSSVTVTSNTWPSQTITCNVAHPASSTKVDKKIESRR |
Light Chain: H
Mutation: NULL
| >6MW9_H|Chain D, H, L, P|EEEV-3 antibody light chain|Mus musculus (10090) QAVVTQESALTTSPGETVTLTCRSNIGAVTSSNCANWVQEKPDHFFTGLIGDTNNRRSGVPARFSGSLIGDKAALTITGAQTEDEAIYFCALWYNNLWVFGGGTKLTVLGQPKSSPSVTLFPPSSEELETNKATLVCTITDFYPGVVTVDWKVDGTPVTQGMETTQPSKQSNNKYMASSYLTLTARAWERHSSYSCQVTHEGHTVEKSLSRADC |
Antigen
Chain: F
Mutation: NULL
| >6MW9_F|Chain B, F, J, N|E2|Eastern equine encephalitis virus (11021) DLDTHFTQYKLARPYIADCPNCGHSRCDSPIAIEEVRGDAHAGVIRIQTSAMFGLKTDGVDLAYMSFMNGKTQKSIKIDNLHVRTSAPCSLVSHHGYYILAQCPPGDTVTVGFHDGPNRHTCTVAHKVEFRPVGREKYRHPPEHGVELPCNRYTHKRADQGHYVEMHQPGLVADHSLLSIHSAKVKITVPSGAQVKYYCKCPDVREGITSSDHTTTCTDVKQCRAYLIDNKKWVYNSGRLPRGEGDTFKGKLHVPFVPVKAKCIATLAPEPLVEHKHRTLILHLHPDHPTLLTTRSLGSDANPTRQWIERPTTVNFTVTGEGLEYTWGNHPPKRVWAQESGEGNPHGWPHEVVVYYYNRYPLTTIIGLCTCVAIIMVSCVTSVWLLCRTRNLCITPYKLAPNAQVPILLALLCCIKPTRA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| G: LEU3050 TYR3052 THR3059 ASP3099 TYR3100 ILE3102 SER3103 H: THR3530 SER3531 SER3532 ASN3533 CYS3534 ASP3552 TRP3593 TYR3594 ASN3595 ASN3596 TRP3598 F: HIS1662 SER1663 LEU1665 SER1666 ILE1667 HIS1668 SER1669 ALA1670 LYS1671 LYS1673 THR1675 SER1698 HIS1700 THR1702 THR1705 ASP1706 VAL1707 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
