Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2493 |
| PDBID: | 6MVL |
| Chains: | HL_A |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 1.61 |
| Reference: | 10.1038/s41586-019-1674-5 |
| Antibody | |
| Antibody: | VISTA.18 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | V-type immunoglobulin domain-containing suppressor of T cell activation |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6MVL_H|Chain B[auth H]|Antibody Fab fragment heavy chain|Homo sapiens (9606) EVQLVESGGGLVQPGKSLRLSCAASGFTLEDYAMHWVRQAPGKGLEWVSGIDWNSENIGYADSVKGRFTISRDNAKNSLYLQMNSLRTEDTALYYCAKVPGYSGGWIDAEDDWGQGTMVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKT |
Light Chain: L
Mutation: NULL
| >6MVL_L|Chain C[auth L]|Antibody Fab fragment light chain|Homo sapiens (9606) EIVLTQSPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSPFTFGPGTKVDIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >6MVL_A|Chain A|V-type immunoglobulin domain-containing suppressor of T-cell activation|Homo sapiens (9606) AFKVATPYSLYVCPEGQNVTLTCRLLGPVDKGHDVTFYKTWYRSSRGEVQTCSERRPIRQLTFQDLHLHHGGHQAAQTSHDLAQRHGLESASDHHGNFSITMRNLTLLDSGLYCCLVVEIRHHHSEHRVHGAMELQVQTGKDAPSNCVVYPSSSQDSEQITAAHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: GLU1 VAL2 GLY26 PHE27 THR28 GLU30 ASP31 TYR32 TRP53 ASN54 GLU56 LYS98 TYR102 SER103 GLY104 GLY105 TRP106 ILE107 ALA109 GLU110 ASP111 ASP112 L: ARG46 THR57 GLY58 A: ASP65 THR67 TYR69 THR71 ARG86 ARG87 PHE94 GLN95 ASP96 HIS98 LEU147 VAL149 ILE151 HIS153 HIS154 HIS155 SER156 GLU157 ARG159 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
