Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2489 |
| PDBID: | 6MUI |
| Chains: | CD_B |
| Organism: | Eastern equine encephalitis virus, Mus musculus |
| Method: | EM |
| Resolution (Å): | 7.70 |
| Reference: | 10.1016/j.celrep.2018.11.067 |
| Antibody | |
| Antibody: | EEEV-42 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | chimeric Eastern Equine Encephalitis Virus E2 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >6MUI_C|Chain C, G, K, O|EEEV-42 antibody heavy chain|Mus musculus (10090) QIQLVQSGREVKNPGETVKISCKASGYTFTEYPMLWVKQAPGKGFRWMGLIYTNTGEPTYAEEFKGRFVFSLEISASTAYLQINNLTNEDTATYFCVRDYFISLDYWGQGTTLTVSSAKTTAPSVYPLAPVCGGTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPALLQSGLYTLSSSVTVTSNTWPSQTITCNVAHPASSTKVDKKIESRR |
Light Chain: D
Mutation: NULL
| >6MUI_D|Chain D, H, L, P|EEEV-42 antibody light chain|Mus musculus (10090) QAVVTQESALTTSPGETVTLTCRSNIGAVTSSNCANWVQEKPDHFFTGLIGDTNNRRSGVPARFSGSLIGDKAALTITGAQTEDEAIYFCALWYNNLWVFGGGTKLTVLGQPKSSPSVTLFPPSSEELETNKATLVCTITDFYPGVVTVDWKVDGTPVTQGMETTQPSKQSNNKYMASSYLTLTARAWERHSSYSCQVTHEGHTVEKSLSRADC |
Antigen
Chain: B
Mutation: NULL
| >6MUI_B|Chain B, F, J, N|E2|Eastern equine encephalitis virus (11021) DLDTHFTQYKLARPYIADCPNCGHSRCDSPIAIEEVRGDAHAGVIRIQTSAMFGLKTDGVDLAYMSFMNGKTQKSIKIDNLHVRTSAPCSLVSHHGYYILAQCPPGDTVTVGFHDGPNRHTCTVAHKVEFRPVGREKYRHPPEHGVELPCNRYTHKRADQGHYVEMHQPGLVADHSLLSIHSAKVKITVPSGAQVKYYCKCPDVREGITSSDHTTTCTDVKQCRAYLIDNKKWVYNSGRLPRGEGDTFKGKLHVPFVPVKAKCIATLAPEPLVEHKHRTLILHLHPDHPTLLTTRSLGSDANPTRQWIERPTTVNFTVTGEGLEYTWGNHPPKRVWAQESGEGNPHGWPHEVVVYYYNRYPLTTIIGLCTCVAIIMVSCVTSVWLLCRTRNLCITPYKLAPNAQVPILLALLCCIKPTRADDTLQVLNYLWNNNQNFFWMQTLIPLAALIVCMRMLRCLFCCGPAFLLVCGALGAAA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: TYR52 GLU57 THR59 ALA61 GLU62 GLU63 LYS65 TYR100 PHE101 ILE102 D: GLN501 ALA502 ILE526 THR530 SER531 SER532 ASN533 CYS534 ASP552 TRP593 TYR594 ASN595 ASN596 LEU597 TRP598 VAL599 B: LYS543 THR544 ASP545 VAL547 TYR551 MET555 GLY557 LYS558 THR559 GLN560 LYS561 SER562 ILE563 SER663 LEU664 THR675 VAL676 PRO677 SER678 GLY679 ALA680 GLN681 ILE695 THR696 SER697 ILE715 ASP716 ASN717 LYS718 LYS719 TRP720 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
