Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2479 |
| PDBID: | 6MN7 |
| Chains: | I_C |
| Organism: | Human immunodeficiency virus 1, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 4.80 |
| Reference: | 10.1038/s41467-019-09481-7 |
| Antibody | |
| Antibody: | BF520.1 scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV-1 BG505.SOSIP.664 gp120 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: I
Mutation: NULL
| >6MN7_I|Chain G, H, I|BF520.1 Fab variable region|Homo sapiens (9606) QVQLVQSGAEMKMPGASVKVSCKASGYTFTGNYIHWVRQAPGQGLEWMGWIAPHSGDTSYAQRFQGRVTMTGDTSLSTAYMELSRLRSDDTAVYYCARGPFPNYYGPGSYWGGLDFWGQGTLVSVSSEIVMTQSPATLSVSLGERATLSCRTSQNVAYNFAWYQQKPGQAPRLLIYEASSRATGTPARFSGSGFGTEFTLTISSMQSEDFAVYYCQQYNNWPSPFTFGPGTKVHIK |
Antigen
Chain: C
Mutation: NULL
| >6MN7_C|Chain A, C, D|Envelope Glycoprotein gp120|Human immunodeficiency virus 1 (11676) AENLWVTVYYGVPVWKDAETTLFCASDAKAYETEKHNVWATHACVPTDPNPQEIHLENVTEEFNMWKNNMVEQMHTDIISLWDQSLKPCVKLTPLCVTLQCTNVTNNITDDMRGELKNCSFNMTTELRDKKQKVYSLFYRLDVVQINENQGNRSNNSNKEYRLINCNTSAITQACPKVSFEPIPIHYCAPAGFAILKCKDKKFNGTGPCPSVSTVQCTHGIKPVVSTQLLLNGSLAEEEVMIRSENITNNAKNILVQFNTPVQINCTRPNNNTRKSIRIGPGQAFYATGDIIGDIRQAHCNVSKATWNETLGKVVKQLRKHFGNNTIIRFANSSGGDLEVTTHSFNCGGEFFYCNTSGLFNSTWISNTSVQGSNSTGSNDSITLPCRIKQIINMWQRIGQAMYAPPIQGVIRCVSNITGLILTRDGGSTNSTTETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRCKRRVVGR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| I: GLN1 TYR27 THR28 THR30 GLY31 ASN32 TYR33 HIS54 PHE101 PRO102 ASN103 TYR104 TRP111 THR183 GLY184 C: ASN137 ILE138 THR139 ASP141 ASN301 ILE323 GLY324 ASP325 ILE326 ARG327 THR415 PRO417 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
