Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2475 |
| PDBID: | 6MLM |
| Chains: | EI_A |
| Organism: | Influenza A virus (A/New York/107/2003(H7N2)), Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.50 |
| Reference: | 10.1371/journal.pbio.3000139 |
| Antibody | |
| Antibody: | H7.5 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus H7N2 hemagglutinin |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >6MLM_E|Chain C[auth E], G[auth F], K|Heavy chain Fv of H7.5 Fab|Homo sapiens (9606) VQLVQSGAEVKKPGASVKVSCKASGYTLTRYYFHWVRQAPGQGFEWMGIINPNGGGTSYAQKFGDRVIMTSDMSTSTIYMELSSLRSEDTAVYYCARDMPYYHDSGGPLFDLWGQGTLVTVSSASTKGPSVFPLAPSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPK |
Light Chain: I
Mutation: NULL
| >6MLM_I|Chain D[auth G], H[auth I], L|Light chain Fv of H7.5 Fab|Homo sapiens (9606) EIVMTQSPSSLSASVGDRVTITCRPSQSISTFLNWYEQKPGKAPKLLIYDASSLQSGVPSRFSGSGSGTEFTLTISSLQPEDFATYYCQQSFSTPYTFGQGTRLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE |
Antigen
Chain: A
Mutation: NULL
| >6MLM_A|Chain A, E[auth B], I[auth H]|Hemagglutinin HA1 chain|Influenza A virus (A/New York/107/2003(H7N2)) (519082) MNTQILAFIACVLTGVKGDKICLGHHAVANGTKVNTLTERGIEVVNATETVETTNIKKICTQGKRPTDLGQCGLLGTLIGPPQCDQFLEFSSDLIIERREGTDICYPGRFTNEESLRQILRRSGGIGKESMGFTYSGIRTNGATSACTRSGSSFYAEMKWLLSNSDNAAFPQMTKAYRNPRNKPALIIWGVHHSESVSEQTKLYGSGNKLITVRSSKYQQSFTPNPGARRIDFHWLLLDPNDTVTFTFNGAFIAPDRTSFFRGESLGVQSDAPLDSSCRGDCFHSGGTIVSSLPFQNINSRTVGKCPRYVKQKSLLLATGMRNVPEKP |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: TYR33 TRP47 ILE50 ASN53 GLY54 GLY55 GLY56 THR57 SER58 TYR59 ALA60 GLN61 GLY64 ASP65 PRO97 I: GLU1 PHE32 PHE92 SER93 THR94 PRO95 TYR96 A: THR142 SER144 GLY145 ASN171 SER172 ASP173 ASN174 ALA175 ALA176 PHE177 PRO178 GLN179 MET180 THR181 LYS182 VAL204 THR208 SER213 GLY214 ASN215 LYS216 LEU217 ARG221 THR262 ASN264 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
